Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor

Sung Eun Hwang, Hyung Min Im, Dong Hoon Kim, Hyun Ju Shin, Dong Hoon Shin, Jeong Eun Park, Inho Jo, Chang Jin Kim, Jong Shin Yoo, Jong Min Kang, Dong Yeol Lim, Sangmee Ahn Jo, Ho Jeong Kwon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

β-amyloid (Aβ) peptides from the proteolytic processing of β-amyloid precursor protein (β-APP) aggregates in the brain to form senile plaques, and their aggregation plays a key role in pathogenesis of Alzheimer's disease (AD). To isolate an active compound that has an Aβ aggregation-inhibitory activity, 2,000 microbial metabolite libraries were screened based on their ability to inhibit Aβ aggregation by using both Congo red and thioflavin T assays. As a result, a water-soluble fraction of a soil microorganism, KK565, showed a potent Aβ aggregation-inhibitory activity. The strain was identified as Streptomyces species, based on the cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. In addition, the purification of active principle resulted in identifying a heat-unstable protein responsible for the Aβ aggregation-inhibitory activity.

Original languageEnglish
Pages (from-to)809-814
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume13
Issue number5
Publication statusPublished - 2003 Oct 1

Fingerprint

Streptomyces
Amyloid
Cultural Characteristics
Diaminopimelic Acid
Congo Red
Aptitude
Amyloid Plaques
Cell Extracts
Cell Wall
Libraries
Alzheimer Disease
Soil
Hot Temperature
Peptides
Water
Brain
Proteins
thioflavin T
Protein Aggregates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Hwang, S. E., Im, H. M., Kim, D. H., Shin, H. J., Shin, D. H., Park, J. E., ... Kwon, H. J. (2003). Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor. Journal of Microbiology and Biotechnology, 13(5), 809-814.
Hwang, Sung Eun ; Im, Hyung Min ; Kim, Dong Hoon ; Shin, Hyun Ju ; Shin, Dong Hoon ; Park, Jeong Eun ; Jo, Inho ; Kim, Chang Jin ; Yoo, Jong Shin ; Kang, Jong Min ; Lim, Dong Yeol ; Jo, Sangmee Ahn ; Kwon, Ho Jeong. / Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor. In: Journal of Microbiology and Biotechnology. 2003 ; Vol. 13, No. 5. pp. 809-814.
@article{246b614cca444c829827bcd76672fd6b,
title = "Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor",
abstract = "β-amyloid (Aβ) peptides from the proteolytic processing of β-amyloid precursor protein (β-APP) aggregates in the brain to form senile plaques, and their aggregation plays a key role in pathogenesis of Alzheimer's disease (AD). To isolate an active compound that has an Aβ aggregation-inhibitory activity, 2,000 microbial metabolite libraries were screened based on their ability to inhibit Aβ aggregation by using both Congo red and thioflavin T assays. As a result, a water-soluble fraction of a soil microorganism, KK565, showed a potent Aβ aggregation-inhibitory activity. The strain was identified as Streptomyces species, based on the cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. In addition, the purification of active principle resulted in identifying a heat-unstable protein responsible for the Aβ aggregation-inhibitory activity.",
author = "Hwang, {Sung Eun} and Im, {Hyung Min} and Kim, {Dong Hoon} and Shin, {Hyun Ju} and Shin, {Dong Hoon} and Park, {Jeong Eun} and Inho Jo and Kim, {Chang Jin} and Yoo, {Jong Shin} and Kang, {Jong Min} and Lim, {Dong Yeol} and Jo, {Sangmee Ahn} and Kwon, {Ho Jeong}",
year = "2003",
month = "10",
day = "1",
language = "English",
volume = "13",
pages = "809--814",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "5",

}

Hwang, SE, Im, HM, Kim, DH, Shin, HJ, Shin, DH, Park, JE, Jo, I, Kim, CJ, Yoo, JS, Kang, JM, Lim, DY, Jo, SA & Kwon, HJ 2003, 'Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor', Journal of Microbiology and Biotechnology, vol. 13, no. 5, pp. 809-814.

Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor. / Hwang, Sung Eun; Im, Hyung Min; Kim, Dong Hoon; Shin, Hyun Ju; Shin, Dong Hoon; Park, Jeong Eun; Jo, Inho; Kim, Chang Jin; Yoo, Jong Shin; Kang, Jong Min; Lim, Dong Yeol; Jo, Sangmee Ahn; Kwon, Ho Jeong.

In: Journal of Microbiology and Biotechnology, Vol. 13, No. 5, 01.10.2003, p. 809-814.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor

AU - Hwang, Sung Eun

AU - Im, Hyung Min

AU - Kim, Dong Hoon

AU - Shin, Hyun Ju

AU - Shin, Dong Hoon

AU - Park, Jeong Eun

AU - Jo, Inho

AU - Kim, Chang Jin

AU - Yoo, Jong Shin

AU - Kang, Jong Min

AU - Lim, Dong Yeol

AU - Jo, Sangmee Ahn

AU - Kwon, Ho Jeong

PY - 2003/10/1

Y1 - 2003/10/1

N2 - β-amyloid (Aβ) peptides from the proteolytic processing of β-amyloid precursor protein (β-APP) aggregates in the brain to form senile plaques, and their aggregation plays a key role in pathogenesis of Alzheimer's disease (AD). To isolate an active compound that has an Aβ aggregation-inhibitory activity, 2,000 microbial metabolite libraries were screened based on their ability to inhibit Aβ aggregation by using both Congo red and thioflavin T assays. As a result, a water-soluble fraction of a soil microorganism, KK565, showed a potent Aβ aggregation-inhibitory activity. The strain was identified as Streptomyces species, based on the cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. In addition, the purification of active principle resulted in identifying a heat-unstable protein responsible for the Aβ aggregation-inhibitory activity.

AB - β-amyloid (Aβ) peptides from the proteolytic processing of β-amyloid precursor protein (β-APP) aggregates in the brain to form senile plaques, and their aggregation plays a key role in pathogenesis of Alzheimer's disease (AD). To isolate an active compound that has an Aβ aggregation-inhibitory activity, 2,000 microbial metabolite libraries were screened based on their ability to inhibit Aβ aggregation by using both Congo red and thioflavin T assays. As a result, a water-soluble fraction of a soil microorganism, KK565, showed a potent Aβ aggregation-inhibitory activity. The strain was identified as Streptomyces species, based on the cultural and morphological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extract. In addition, the purification of active principle resulted in identifying a heat-unstable protein responsible for the Aβ aggregation-inhibitory activity.

UR - http://www.scopus.com/inward/record.url?scp=0242551359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242551359&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 809

EP - 814

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 5

ER -

Hwang SE, Im HM, Kim DH, Shin HJ, Shin DH, Park JE et al. Isolation of Streptomyces sp. KK65 as a produceer of β-amyloid aggregation inhibitor. Journal of Microbiology and Biotechnology. 2003 Oct 1;13(5):809-814.