Isoliquiritigenin (ISL) inhibits ErbB3 signaling in prostate cancer cells

Jae In Jung, Eunkyung Chung, Mi Ra Seon, Hyun Kyung Shin, Eun Ji Kim, Soon Sung Lim, Won Yoon Chung, Kwang Kyun Park, Jung Han Yoon Park

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48 Citations (Scopus)

Abstract

Isoliquiritigenin (ISL), a flavonoid found in licorice, shallot, and bean sprouts, has been identified as a potent anti-tumor promoting agent. We previously demonstrated that ISL reduces cell proliferation and induces apoptosis in DU145 human prostate cancer cells and MAT-LyLu (MLL) rat prostate cancer cells. Overexpression of members of the ErbB receptor family is a frequently observed event in several human cancers, and ErbB receptors currently constitute the primary targets of anticancer strategies. In order to elucidate the mechanisms underlying the ISL regulation of prostate cancer cell proliferation, the present study attempted to determine whether ISL inhibits heregulin (HRG)-β-induced ErbB3 signaling. DU145 and MLL cells were cultured in serum-free medium with ISL and/or HRG-β. Exogenous HRG-β alone was shown to effect an increase in the numbers of viable cells, whereas HRG-β did not counteract the ISL-induced growth inhibition. ISL reduced the protein and mRNA levels of ErbB3 in a dose-dependent manner, but exerted no effect on HRG protein levels. Immunoprecipitation/Western blot studies indicated that ISL inhibited the HRG-β-induced tyrosine phosphorylation of ErbB3, the recruitment of the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K) to ErbB3, and Akt phosphorylation in DU145 cells. These results indicate that ISL inhibits the proliferation of prostate cancer cells, at least in part, via the inhibition of ErbB3 signaling and the PI3K/Akt pathway.

Original languageEnglish
Pages (from-to)159-168
Number of pages10
JournalBioFactors
Volume28
Issue number3-4
DOIs
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

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    Jung, J. I., Chung, E., Seon, M. R., Shin, H. K., Kim, E. J., Lim, S. S., Chung, W. Y., Park, K. K., & Park, J. H. Y. (2006). Isoliquiritigenin (ISL) inhibits ErbB3 signaling in prostate cancer cells. BioFactors, 28(3-4), 159-168. https://doi.org/10.1002/biof.5520280302