Ixeris dentata extract increases salivary secretion through the regulation of endoplasmic reticulum stress in a diabetes-induced xerostomia rat model

Kashi Raj Bhattarai, Hwa Young Lee, Seung Hyun Kim, Hyung Ryong Kim, Han Jung Chae

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study aimed to investigate the molecular mechanism of diabetes mellitus (DM)-induced dry mouth and an application of natural products from Ixeris dentata (IXD), a recently suggested regulator of amylase secretion in salivary cells. Vehicle-treated or diabetic rats were orally treated with either water or an IXD extract for 10 days to observe the effect on salivary flow. We found that the IXD extract increased aquaporin 5 (AQP5) and alpha-amylase protein expression in the submandibular gland along with salivary flow rate. Similarly, the IXD extract and its purified compound increased amylase secretion in high glucose-exposed human salivary gland cells. Furthermore, increased endoplasmic reticulum stress response in the submandibular gland of diabetic rats was inhibited by treatment with the IXD extract, suggesting that IXD extract treatment improves the ER environment by increasing the protein folding capacity. Thus, pharmacological treatment with the IXD extract is suggested to relieve DM-induced dry mouth symptoms.

Original languageEnglish
Article number1059
JournalInternational journal of molecular sciences
Volume19
Issue number4
DOIs
Publication statusPublished - 2018 Apr

Fingerprint

endoplasmic reticulum
Xerostomia
Amylases
secretions
Asteraceae
Endoplasmic Reticulum Stress
Medical problems
diabetes mellitus
rats
Rats
glands
Aquaporin 5
mouth
Protein folding
alpha-Amylases
Biological Products
proteins
salivary glands
regulators
Flow rate

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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abstract = "This study aimed to investigate the molecular mechanism of diabetes mellitus (DM)-induced dry mouth and an application of natural products from Ixeris dentata (IXD), a recently suggested regulator of amylase secretion in salivary cells. Vehicle-treated or diabetic rats were orally treated with either water or an IXD extract for 10 days to observe the effect on salivary flow. We found that the IXD extract increased aquaporin 5 (AQP5) and alpha-amylase protein expression in the submandibular gland along with salivary flow rate. Similarly, the IXD extract and its purified compound increased amylase secretion in high glucose-exposed human salivary gland cells. Furthermore, increased endoplasmic reticulum stress response in the submandibular gland of diabetic rats was inhibited by treatment with the IXD extract, suggesting that IXD extract treatment improves the ER environment by increasing the protein folding capacity. Thus, pharmacological treatment with the IXD extract is suggested to relieve DM-induced dry mouth symptoms.",
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Ixeris dentata extract increases salivary secretion through the regulation of endoplasmic reticulum stress in a diabetes-induced xerostomia rat model. / Bhattarai, Kashi Raj; Lee, Hwa Young; Kim, Seung Hyun; Kim, Hyung Ryong; Chae, Han Jung.

In: International journal of molecular sciences, Vol. 19, No. 4, 1059, 04.2018.

Research output: Contribution to journalArticle

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AU - Chae, Han Jung

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