KETCH1 imports HYL1 to nucleus for miRNA biogenesis in Arabidopsis

Zhonghui Zhang, Xinwei Guo, Chunxiao Ge, Zeyang Ma, Mengqiu Jiang, Tianhong Li, Hisashi Koiwa, Seong Wook Yang, Xiuren Zhang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

MicroRNA (miRNA) is processed from primary transcripts with hairpin structures (pri-miRNAs) by microprocessors in the nucleus. How cytoplasmic-borne microprocessor components are transported into the nucleus to fulfill their functions remains poorly understood. Here, we report KETCH1 (karyopherin enabling the transport of the cytoplasmic HYL1) as a partner of hyponastic leaves 1 (HYL1) protein, a core component of microprocessor in Arabidopsis and functional counterpart of DGCR8/Pasha in animals. Null mutation of ketch1 is embryonic-lethal, whereas knockdown mutation of ketch1 caused morphological defects, reminiscent of mutants in the miRNA pathway. ketch1 knockdown mutation also substantially reduced miRNA accumulation, but did not alter nuclear-cytoplasmic shuttling of miRNAs. Rather, the mutation significantly reduced nuclear portion of HYL1 protein and correspondingly compromised the pri-miRNA processing in the nucleus. We propose that KETCH1 transports HYL1 from the cytoplasm to the nucleus to constitute functional microprocessor in Arabidopsis. This study provides insight into the largely unknown nuclear-cytoplasmic trafficking process of miRNA biogenesis components through eukaryotes.

Original languageEnglish
Pages (from-to)4011-4016
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number15
DOIs
Publication statusPublished - 2017 Apr 11

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Karyopherins
MicroRNAs
Arabidopsis
Microcomputers
Mutation
Eukaryota
Cytoplasm
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Zhang, Zhonghui ; Guo, Xinwei ; Ge, Chunxiao ; Ma, Zeyang ; Jiang, Mengqiu ; Li, Tianhong ; Koiwa, Hisashi ; Yang, Seong Wook ; Zhang, Xiuren. / KETCH1 imports HYL1 to nucleus for miRNA biogenesis in Arabidopsis. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 15. pp. 4011-4016.
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KETCH1 imports HYL1 to nucleus for miRNA biogenesis in Arabidopsis. / Zhang, Zhonghui; Guo, Xinwei; Ge, Chunxiao; Ma, Zeyang; Jiang, Mengqiu; Li, Tianhong; Koiwa, Hisashi; Yang, Seong Wook; Zhang, Xiuren.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 15, 11.04.2017, p. 4011-4016.

Research output: Contribution to journalArticle

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AU - Koiwa, Hisashi

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AU - Zhang, Xiuren

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