Arabidopsis development proceeds from three stem cell populations located at the shoot, flower, and root meristems. The relationship between the highly related shoot and flower stem cells and the very divergent root stem cells has been unclear. We show that the related phosphatases POL and PLL1 are required for all three stem cell populations. pol pll1 mutant embryos lack key asymmetric divisions that give rise to the root stem cell organizer and the central vascular axis. Instead, these cells divide in a superficially symmetric fashion in pol pll1 embryos, leading to a loss of embryonic and postembryonic root stem cells and vascular specification. We present data that show that POL/PLL1 drive root stem cell specification by promoting expression of the WUS homolog WOX5. We propose that POL and PLL1 are required for the proper divisions of shoot, flower, and root stem cell organizers, WUS/WOX5 gene expression, and stem cell maintenance.
Bibliographical noteFunding Information:
This work was supported by a grant from the National Institutes of Health (R01GM62962) to S.E.C., by the Crop Functional Genomics Center of the 21 st Century Frontier Program (Code No. CG2152), and by Korea Research Foundation (C00469) grants to M.M.L. Soochin Cho assisted in optimizing embryo microscopy. Michael Prigge, Su-Hwan Kwak, John Schiefelbein, Philip Benfey, and Thomas Laux kindly provided key reagents.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
- Cell Biology