Kinetics of CO2 fixation by Sulfurovum lithotrophicum 42BKTT: Medium optimization and tolerance of CO2 toxicity

Sewon Park, Hyuk Sung Kwon, Chang Ha Lee, Ik Sung Ahn

Research output: Contribution to journalArticlepeer-review

Abstract

CO2 toxicity, ionic strength, and specific culture media components, such as thiosulfate have been reported to influence the growth of CO2-fixing autotrophic bacteria. In this study, a chemostat culture was used to determine the kinetics of CO2 fixation by S. lithotrophicum 42BKTT. The medium composition was determined to be capable of reaching a steady state at a high concentration of thiosulfate, an energy source, without growth inhibition by the excessively high ionic strength and thiosulfate itself. CO2 was supplied by saturating the medium with a 1 atm CO2 gas or a gaseous mixture of 0.8 atm N2 and 0.2 atm CO2. The Monod equation successfully described the kinetics of CO2 fixation; the maximum specific growth rate was 0.33 h−1 regardless of CO2 pressure while the Monod constant was 0.0379 g Na2S2O3/L and 0.196 g Na2S2O3/L under 0.2 atm and 1.0 atm CO2, respectively. We found that when nutrients, including thiosulfate, were supplied sufficiently, the CO2 fixation rate at 1 atm CO2 was only slightly lower than that at 0.2 atm CO2 (e.g. only by 4.7 % for 3.0 g Na2S2O3/L). Therefore, the CO2 fixation by S. lithotrophicum 42BKTT is not subject to CO2 toxicity at least up to 1.0 atm CO2.

Original languageEnglish
Article number104428
JournalJournal of Environmental Chemical Engineering
Volume8
Issue number6
DOIs
Publication statusPublished - 2020 Dec

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Waste Management and Disposal
  • Pollution
  • Process Chemistry and Technology

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