Kinetics of LCFA Inhibition on Acetoclastic Methanogenesis, Propionate Degradation and β-Oxidation

Sang Hyoun Kim, Sun Kee Han, Hang Sik Shin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Kinetics of long-chain fatty acids (LCFAs) inhibition on acetoclastic methanogenesis, propionate degradation and β-oxidation were studied with granular sludge under mesophilic batch conditions. Mathematical expressions used for reaction rates were as shown below: Equation presented: The simulated results revealed that the methane production rates from acetate decreased with an increase in both concentration and the number of double bonds of LCFAs. The concentrations of oleate (C18:1), linoleate (C18:2), palmitate (C16:0), and stearate (C18:0) were 0.54mM, 0.11 mM 1.62 mM, and 2.58 mM, respectively, at which the methane production rates from acetate dropped 10%, and 3.10 mM, 0.72 mM, 5.71 mM, and 5.37 mM, respectively, at which the rates dropped 50%. The inhibitory effects of LCFAs on propionate degradation showed a similar tendency with acetoclastic methanogenesis; however, were less severe. The concentrations of oleate, linoleate, palmitate, and stearate were 1.02 mM, 0.18 mM, 2.34 mM, and 1.92 mM, respectively, at which the propionate degradation rates dropped 10%, and 4.38 mM, 1.17 mM, 5.88 mM, and 5.18 mM, respectively, at which the rates dropped 50%. The observed maximum βoxidation rates of oleate, linoleate, palmitate, and stearate were 0.21 mmol (gVSS)-1d -1, 0.09 mmol (gVSS)-1d-1, 0.12 mmol (gVSS)-1d-1, and 0.08 mmol (gVSS)-1d -1, respectively. The lag-phase times in β-oxidation were also dependent on LCFA concentrations. The concentrations of oleate, linoleate, palmitate, and stearate, at which the lag-phase times became 5 days, were 5.93 mM, 2.24 mM, 4.02 mM, and 2.81 mM, respectively.

Original languageEnglish
Pages (from-to)1025-1037
Number of pages13
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume39
Issue number4
DOIs
Publication statusPublished - 2004 Apr 1

Fingerprint

Stearates
Palmitates
Propionates
methanogenesis
Linoleic Acid
Oleic Acid
Fatty acids
Fatty Acids
fatty acid
oxidation
Degradation
kinetics
Oxidation
degradation
Kinetics
Methane
Acetates
acetate
methane
Reaction rates

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

@article{e1e72c7af48f4d96885d77d29645edec,
title = "Kinetics of LCFA Inhibition on Acetoclastic Methanogenesis, Propionate Degradation and β-Oxidation",
abstract = "Kinetics of long-chain fatty acids (LCFAs) inhibition on acetoclastic methanogenesis, propionate degradation and β-oxidation were studied with granular sludge under mesophilic batch conditions. Mathematical expressions used for reaction rates were as shown below: Equation presented: The simulated results revealed that the methane production rates from acetate decreased with an increase in both concentration and the number of double bonds of LCFAs. The concentrations of oleate (C18:1), linoleate (C18:2), palmitate (C16:0), and stearate (C18:0) were 0.54mM, 0.11 mM 1.62 mM, and 2.58 mM, respectively, at which the methane production rates from acetate dropped 10{\%}, and 3.10 mM, 0.72 mM, 5.71 mM, and 5.37 mM, respectively, at which the rates dropped 50{\%}. The inhibitory effects of LCFAs on propionate degradation showed a similar tendency with acetoclastic methanogenesis; however, were less severe. The concentrations of oleate, linoleate, palmitate, and stearate were 1.02 mM, 0.18 mM, 2.34 mM, and 1.92 mM, respectively, at which the propionate degradation rates dropped 10{\%}, and 4.38 mM, 1.17 mM, 5.88 mM, and 5.18 mM, respectively, at which the rates dropped 50{\%}. The observed maximum βoxidation rates of oleate, linoleate, palmitate, and stearate were 0.21 mmol (gVSS)-1d -1, 0.09 mmol (gVSS)-1d-1, 0.12 mmol (gVSS)-1d-1, and 0.08 mmol (gVSS)-1d -1, respectively. The lag-phase times in β-oxidation were also dependent on LCFA concentrations. The concentrations of oleate, linoleate, palmitate, and stearate, at which the lag-phase times became 5 days, were 5.93 mM, 2.24 mM, 4.02 mM, and 2.81 mM, respectively.",
author = "Kim, {Sang Hyoun} and Han, {Sun Kee} and Shin, {Hang Sik}",
year = "2004",
month = "4",
day = "1",
doi = "10.1081/ESE-120028411",
language = "English",
volume = "39",
pages = "1025--1037",
journal = "Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering",
issn = "1093-4529",
publisher = "Taylor and Francis Ltd.",
number = "4",

}

TY - JOUR

T1 - Kinetics of LCFA Inhibition on Acetoclastic Methanogenesis, Propionate Degradation and β-Oxidation

AU - Kim, Sang Hyoun

AU - Han, Sun Kee

AU - Shin, Hang Sik

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Kinetics of long-chain fatty acids (LCFAs) inhibition on acetoclastic methanogenesis, propionate degradation and β-oxidation were studied with granular sludge under mesophilic batch conditions. Mathematical expressions used for reaction rates were as shown below: Equation presented: The simulated results revealed that the methane production rates from acetate decreased with an increase in both concentration and the number of double bonds of LCFAs. The concentrations of oleate (C18:1), linoleate (C18:2), palmitate (C16:0), and stearate (C18:0) were 0.54mM, 0.11 mM 1.62 mM, and 2.58 mM, respectively, at which the methane production rates from acetate dropped 10%, and 3.10 mM, 0.72 mM, 5.71 mM, and 5.37 mM, respectively, at which the rates dropped 50%. The inhibitory effects of LCFAs on propionate degradation showed a similar tendency with acetoclastic methanogenesis; however, were less severe. The concentrations of oleate, linoleate, palmitate, and stearate were 1.02 mM, 0.18 mM, 2.34 mM, and 1.92 mM, respectively, at which the propionate degradation rates dropped 10%, and 4.38 mM, 1.17 mM, 5.88 mM, and 5.18 mM, respectively, at which the rates dropped 50%. The observed maximum βoxidation rates of oleate, linoleate, palmitate, and stearate were 0.21 mmol (gVSS)-1d -1, 0.09 mmol (gVSS)-1d-1, 0.12 mmol (gVSS)-1d-1, and 0.08 mmol (gVSS)-1d -1, respectively. The lag-phase times in β-oxidation were also dependent on LCFA concentrations. The concentrations of oleate, linoleate, palmitate, and stearate, at which the lag-phase times became 5 days, were 5.93 mM, 2.24 mM, 4.02 mM, and 2.81 mM, respectively.

AB - Kinetics of long-chain fatty acids (LCFAs) inhibition on acetoclastic methanogenesis, propionate degradation and β-oxidation were studied with granular sludge under mesophilic batch conditions. Mathematical expressions used for reaction rates were as shown below: Equation presented: The simulated results revealed that the methane production rates from acetate decreased with an increase in both concentration and the number of double bonds of LCFAs. The concentrations of oleate (C18:1), linoleate (C18:2), palmitate (C16:0), and stearate (C18:0) were 0.54mM, 0.11 mM 1.62 mM, and 2.58 mM, respectively, at which the methane production rates from acetate dropped 10%, and 3.10 mM, 0.72 mM, 5.71 mM, and 5.37 mM, respectively, at which the rates dropped 50%. The inhibitory effects of LCFAs on propionate degradation showed a similar tendency with acetoclastic methanogenesis; however, were less severe. The concentrations of oleate, linoleate, palmitate, and stearate were 1.02 mM, 0.18 mM, 2.34 mM, and 1.92 mM, respectively, at which the propionate degradation rates dropped 10%, and 4.38 mM, 1.17 mM, 5.88 mM, and 5.18 mM, respectively, at which the rates dropped 50%. The observed maximum βoxidation rates of oleate, linoleate, palmitate, and stearate were 0.21 mmol (gVSS)-1d -1, 0.09 mmol (gVSS)-1d-1, 0.12 mmol (gVSS)-1d-1, and 0.08 mmol (gVSS)-1d -1, respectively. The lag-phase times in β-oxidation were also dependent on LCFA concentrations. The concentrations of oleate, linoleate, palmitate, and stearate, at which the lag-phase times became 5 days, were 5.93 mM, 2.24 mM, 4.02 mM, and 2.81 mM, respectively.

UR - http://www.scopus.com/inward/record.url?scp=2342425739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342425739&partnerID=8YFLogxK

U2 - 10.1081/ESE-120028411

DO - 10.1081/ESE-120028411

M3 - Article

VL - 39

SP - 1025

EP - 1037

JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

SN - 1093-4529

IS - 4

ER -