KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

Ki Duk Song; Duk Jung Kim; Jong Eun Lee; Cheol Heui Yun; Woon Kyu Lee

doi:10.1016/j.bbrc.2012.02.032

KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

Ki Duk Song, Duk Jung Kim, Jong Eun Lee, Cheol Heui Yun, Woon Kyu Lee

Department of Anatomy

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Krüppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21^WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21^WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21^WAF1/CIP1 transcription.

Original language	English
Pages (from-to)	388-394
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	419
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2012.02.032
Publication status	Published - 2012 Mar 9

Bibliographical note

Funding Information:
This work was supported by a Grant (Code # 20070401034031 ) from BioGreen 21 Program, Rural Development Administration, Republic of Korea and INHA University Research Grant.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2012.02.032

Cite this

@article{da9100b5ecc74a77b48ded8697700de9,

title = "KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor",

abstract = "Kr{\"u}ppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21WAF1/CIP1 transcription.",

author = "Song, {Ki Duk} and Kim, {Duk Jung} and Lee, {Jong Eun} and Yun, {Cheol Heui} and Lee, {Woon Kyu}",

note = "Funding Information: This work was supported by a Grant (Code # 20070401034031 ) from BioGreen 21 Program, Rural Development Administration, Republic of Korea and INHA University Research Grant.",

year = "2012",

month = mar,

day = "9",

doi = "10.1016/j.bbrc.2012.02.032",

language = "English",

volume = "419",

pages = "388--394",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

AU - Song, Ki Duk

AU - Kim, Duk Jung

AU - Lee, Jong Eun

AU - Yun, Cheol Heui

AU - Lee, Woon Kyu

N1 - Funding Information: This work was supported by a Grant (Code # 20070401034031 ) from BioGreen 21 Program, Rural Development Administration, Republic of Korea and INHA University Research Grant.

PY - 2012/3/9

Y1 - 2012/3/9

N2 - Krüppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21WAF1/CIP1 transcription.

AB - Krüppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21WAF1/CIP1 transcription, which was independent of p53 and Sp1 binding sites in p21WAF1/CIP1 promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21WAF1/CIP1 transcription.

UR - http://www.scopus.com/inward/record.url?scp=84862804986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862804986&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2012.02.032

DO - 10.1016/j.bbrc.2012.02.032

M3 - Article

C2 - 22349513

AN - SCOPUS:84862804986

SN - 0006-291X

VL - 419

SP - 388

EP - 394

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

KLF10, transforming growth factor-β-inducible early gene 1, acts as a tumor suppressor

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this