Knockdown of moesin expression accelerates cellular senescence of human dermal microvascular endothelial cells

JuHee Lee, Jung Hoan Yoo, SangHo Oh, Kyu Yeop Lee, Kwanghoon Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: Endothelial cells maintain the homeostasis of blood, which consists of plasma and cellular components, and regulate the interaction between blood and the surrounding tissues. They also have essential roles in vascular permeability, the circulation, coagulation, inflammation, wound healing, and tissue growth. The senescence of endothelial cells is closely related to the aging of the adjacent tissues and to age-related vascular disease. Recently, the expression of moesin was found to be decreased in elderly human dermal microvascular endothelial cells (HDMECs), and an association between moesin and senescence has been suggested. This study examined the functional role of moesin in cellular senescence. Materials and Methods: To study the effects of decreased moesin expression on cellular senescence and metabolism, HDMECs were transfected with short hairpin-RNA (shRNA) lentivirus to silence moesin gene expression. In addition, specimens from young and old human skin were stained with antimoesin and anti-p16 antibodies as an in vivo study. Results: Using shRNAlentivirus, moesin knock-down HDMECs developed characteristics associated with aging and expressed senescence associated-beta-galactosidase during early passages. They also showed increased p16 expression, decreased metabolic activity, and cell growth retardation. Human skin tissue from elderly persons showed decreased moesin expression and increased p16 expression. Conclusion: These findings suggest that there is a functional association between moesin expression and cellular senescence. Further study of the functional mechanism of moesin in the cytoskeleton and cellular senescence is needed. In addition, this study provides a useful model for developing anti-aging treatments.

Original languageEnglish
Pages (from-to)438-447
Number of pages10
JournalYonsei medical journal
Volume51
Issue number3
DOIs
Publication statusPublished - 2010 May 1

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Cell Aging
Endothelial Cells
Skin
moesin
Lentivirus
Capillary Permeability
beta-Galactosidase
Growth
Cytoskeleton
Vascular Diseases
Wound Healing
Small Interfering RNA
Anti-Idiotypic Antibodies
Homeostasis
Inflammation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Knockdown of moesin expression accelerates cellular senescence of human dermal microvascular endothelial cells",
abstract = "Purpose: Endothelial cells maintain the homeostasis of blood, which consists of plasma and cellular components, and regulate the interaction between blood and the surrounding tissues. They also have essential roles in vascular permeability, the circulation, coagulation, inflammation, wound healing, and tissue growth. The senescence of endothelial cells is closely related to the aging of the adjacent tissues and to age-related vascular disease. Recently, the expression of moesin was found to be decreased in elderly human dermal microvascular endothelial cells (HDMECs), and an association between moesin and senescence has been suggested. This study examined the functional role of moesin in cellular senescence. Materials and Methods: To study the effects of decreased moesin expression on cellular senescence and metabolism, HDMECs were transfected with short hairpin-RNA (shRNA) lentivirus to silence moesin gene expression. In addition, specimens from young and old human skin were stained with antimoesin and anti-p16 antibodies as an in vivo study. Results: Using shRNAlentivirus, moesin knock-down HDMECs developed characteristics associated with aging and expressed senescence associated-beta-galactosidase during early passages. They also showed increased p16 expression, decreased metabolic activity, and cell growth retardation. Human skin tissue from elderly persons showed decreased moesin expression and increased p16 expression. Conclusion: These findings suggest that there is a functional association between moesin expression and cellular senescence. Further study of the functional mechanism of moesin in the cytoskeleton and cellular senescence is needed. In addition, this study provides a useful model for developing anti-aging treatments.",
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Knockdown of moesin expression accelerates cellular senescence of human dermal microvascular endothelial cells. / Lee, JuHee; Yoo, Jung Hoan; Oh, SangHo; Lee, Kyu Yeop; Lee, Kwanghoon.

In: Yonsei medical journal, Vol. 51, No. 3, 01.05.2010, p. 438-447.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Knockdown of moesin expression accelerates cellular senescence of human dermal microvascular endothelial cells

AU - Lee, JuHee

AU - Yoo, Jung Hoan

AU - Oh, SangHo

AU - Lee, Kyu Yeop

AU - Lee, Kwanghoon

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N2 - Purpose: Endothelial cells maintain the homeostasis of blood, which consists of plasma and cellular components, and regulate the interaction between blood and the surrounding tissues. They also have essential roles in vascular permeability, the circulation, coagulation, inflammation, wound healing, and tissue growth. The senescence of endothelial cells is closely related to the aging of the adjacent tissues and to age-related vascular disease. Recently, the expression of moesin was found to be decreased in elderly human dermal microvascular endothelial cells (HDMECs), and an association between moesin and senescence has been suggested. This study examined the functional role of moesin in cellular senescence. Materials and Methods: To study the effects of decreased moesin expression on cellular senescence and metabolism, HDMECs were transfected with short hairpin-RNA (shRNA) lentivirus to silence moesin gene expression. In addition, specimens from young and old human skin were stained with antimoesin and anti-p16 antibodies as an in vivo study. Results: Using shRNAlentivirus, moesin knock-down HDMECs developed characteristics associated with aging and expressed senescence associated-beta-galactosidase during early passages. They also showed increased p16 expression, decreased metabolic activity, and cell growth retardation. Human skin tissue from elderly persons showed decreased moesin expression and increased p16 expression. Conclusion: These findings suggest that there is a functional association between moesin expression and cellular senescence. Further study of the functional mechanism of moesin in the cytoskeleton and cellular senescence is needed. In addition, this study provides a useful model for developing anti-aging treatments.

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