L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor

Sung Hae Bae, Hoon Ryu, Kijong Rhee, Ji Eun Oh, Soonkoo Baik, Kwang Yong Shim, Jee Hyun Kong, Shin Young Hyun, Hyun Sung Pack, Changjo Im, Ha Cheol Shin, Yong Man Kim, Hyun Soo Kim, Young Woo Eom, Jong In Lee

Research output: Contribution to journalArticle

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Abstract

l-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess "stemness" during long-term culture.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalGrowth Factors
Volume33
Issue number2
DOIs
Publication statusPublished - 2015 Apr 1

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Hepatocyte Growth Factor
Fibroblast Growth Factor 2
Stem cells
Mesenchymal Stromal Cells
Bone
Bone Marrow
Fibroblast Growth Factor 4
Serial Passage
Autophagy
ascorbate-2-phosphate
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Down-Regulation
Antioxidants

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Bae, Sung Hae ; Ryu, Hoon ; Rhee, Kijong ; Oh, Ji Eun ; Baik, Soonkoo ; Shim, Kwang Yong ; Kong, Jee Hyun ; Hyun, Shin Young ; Pack, Hyun Sung ; Im, Changjo ; Shin, Ha Cheol ; Kim, Yong Man ; Kim, Hyun Soo ; Eom, Young Woo ; Lee, Jong In. / L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. In: Growth Factors. 2015 ; Vol. 33, No. 2. pp. 71-78.
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L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. / Bae, Sung Hae; Ryu, Hoon; Rhee, Kijong; Oh, Ji Eun; Baik, Soonkoo; Shim, Kwang Yong; Kong, Jee Hyun; Hyun, Shin Young; Pack, Hyun Sung; Im, Changjo; Shin, Ha Cheol; Kim, Yong Man; Kim, Hyun Soo; Eom, Young Woo; Lee, Jong In.

In: Growth Factors, Vol. 33, No. 2, 01.04.2015, p. 71-78.

Research output: Contribution to journalArticle

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AU - Hyun, Shin Young

AU - Pack, Hyun Sung

AU - Im, Changjo

AU - Shin, Ha Cheol

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AU - Kim, Hyun Soo

AU - Eom, Young Woo

AU - Lee, Jong In

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