Abstract
For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)6]3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL-1 and 1000ngmL-1 range and associated with a detection limits of 0.06 and 0.08ngmL-1 respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 37-44 |
| Number of pages | 8 |
| Journal | Bioelectrochemistry |
| Volume | 107 |
| DOIs | |
| Publication status | Published - 2016 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Physical and Theoretical Chemistry
- Electrochemistry
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Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor. / Yagati, Ajay Kumar; Pyun, Jae Chul; Min, Junhong; Cho, Sungbo.
In: Bioelectrochemistry, Vol. 107, 01.02.2016, p. 37-44.Research output: Contribution to journal › Article
TY - JOUR
T1 - Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor
AU - Yagati, Ajay Kumar
AU - Pyun, Jae Chul
AU - Min, Junhong
AU - Cho, Sungbo
PY - 2016/2/1
Y1 - 2016/2/1
N2 - For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)6]3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL-1 and 1000ngmL-1 range and associated with a detection limits of 0.06 and 0.08ngmL-1 respectively.
AB - For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)6]3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL-1 and 1000ngmL-1 range and associated with a detection limits of 0.06 and 0.08ngmL-1 respectively.
UR - http://www.scopus.com/inward/record.url?scp=84944462953&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84944462953&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2015.10.002
DO - 10.1016/j.bioelechem.2015.10.002
M3 - Article
C2 - 26523504
AN - SCOPUS:84944462953
VL - 107
SP - 37
EP - 44
JO - Bioelectrochemistry
JF - Bioelectrochemistry
SN - 1567-5394
ER -