Label-free cell-based assay with spectral-domain optical coherence phase microscopy

Suho Ryu, Kyung A. Hyun, Jung Heo, Hyo Il Jung, Chulmin Joo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Quantitative measurement of dynamic responses of unstained living cells is of great importance in many applications ranging from investigation of fundamental cellular functions to drug discoveries. Conventional optical methods for label-free cell-based assay examine cellular structural changes proximal to sensor surfaces under external stimuli, but require dedicated nanostructure-patterned substrates for operation. Here, we present a quantitative imaging method, spectral-domain optical coherence phase microscopy (SD-OCPM), as a viable optical platform for label-free cell-based assay. The instrument is based on a low-coherence interferometric microscope that enables quantitative depth-resolved phase measurement of a transparent specimen with high phase stability. We demonstrate SD-OCPM measurement of dynamic responses of human breast cancer cells (MCF-7) to 2-picolinic acid (PA) and histamine.

Original languageEnglish
Article number046003
JournalJournal of Biomedical Optics
Volume19
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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phase coherence
Labels
Assays
Microscopic examination
microscopy
dynamic response
Dynamic response
cells
Cells
histamines
Phase measurement
Phase stability
spectral methods
breast
stimuli
Histamine
Nanostructures
drugs
Microscopes
platforms

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

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Label-free cell-based assay with spectral-domain optical coherence phase microscopy. / Ryu, Suho; Hyun, Kyung A.; Heo, Jung; Jung, Hyo Il; Joo, Chulmin.

In: Journal of Biomedical Optics, Vol. 19, No. 4, 046003, 01.01.2014.

Research output: Contribution to journalArticle

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