Label-free impedimetric glycosensor based on β-galactose-functionalized gold electrode for the determination of cholera toxin

Daeho Jeong, Kwang Soo Ahn, Wonyong Lee

Research output: Contribution to journalArticle

Abstract

A label-free impedimetric glycosensor has been developed for the direct detection of cholera toxin (CT). The glycosensor has been fabricated by forming the self-assembled monolayers of β-galactose derivatives containing poly-ethyleneglycol (PEG) spacer on gold electrode surfaces. The selective binding of CT to the β-galactose receptors on the fabricated glycosensor has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. gmonolayers on gold electrode surface has been determined to be ca. 2.53 × 1010 M− 1, indicating that the present glycosensor can selectively capture the target CT from sample solutions. Therefore, the present monosaccharide β-galactose derivative can be used as a receptor for CT as an alternative to CT-specific antibodies or pentasaccharide ganglioside GM1, most widely used in conventional CT assays. The present glycosensor can detect CT in the concentration range from 1.18 × 10− 12 M to 1.18 × 10− 9 M (r2 = 0.963) with a limit of detection (S/N = 3) down to 7.83 × 10− 13 M, which is much lower than those obtained with other detection methods. Since the present glycosensor-based CT assay does not require complex and time-consuming signal amplification steps generally employed in sandwich-type immunoassays, it enables the simple and rapid detection of CT.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalJournal of Electroanalytical Chemistry
Volume806
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Cholera Toxin
Galactose
Gold
Labels
Assays
Derivatives
Electrodes
Self assembled monolayers
Electrochemical impedance spectroscopy
Antibodies
Amplification
G(M1) Ganglioside
Monosaccharides
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

Cite this

@article{3c3dc3ee204f4ef1822434bbafe2ed4b,
title = "Label-free impedimetric glycosensor based on β-galactose-functionalized gold electrode for the determination of cholera toxin",
abstract = "A label-free impedimetric glycosensor has been developed for the direct detection of cholera toxin (CT). The glycosensor has been fabricated by forming the self-assembled monolayers of β-galactose derivatives containing poly-ethyleneglycol (PEG) spacer on gold electrode surfaces. The selective binding of CT to the β-galactose receptors on the fabricated glycosensor has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. gmonolayers on gold electrode surface has been determined to be ca. 2.53 × 1010 M− 1, indicating that the present glycosensor can selectively capture the target CT from sample solutions. Therefore, the present monosaccharide β-galactose derivative can be used as a receptor for CT as an alternative to CT-specific antibodies or pentasaccharide ganglioside GM1, most widely used in conventional CT assays. The present glycosensor can detect CT in the concentration range from 1.18 × 10− 12 M to 1.18 × 10− 9 M (r2 = 0.963) with a limit of detection (S/N = 3) down to 7.83 × 10− 13 M, which is much lower than those obtained with other detection methods. Since the present glycosensor-based CT assay does not require complex and time-consuming signal amplification steps generally employed in sandwich-type immunoassays, it enables the simple and rapid detection of CT.",
author = "Daeho Jeong and Ahn, {Kwang Soo} and Wonyong Lee",
year = "2017",
month = "12",
day = "1",
doi = "10.1016/j.jelechem.2017.10.064",
language = "English",
volume = "806",
pages = "123--129",
journal = "Journal of Electroanalytical Chemistry",
issn = "0368-1874",
publisher = "Elsevier Sequoia",

}

Label-free impedimetric glycosensor based on β-galactose-functionalized gold electrode for the determination of cholera toxin. / Jeong, Daeho; Ahn, Kwang Soo; Lee, Wonyong.

In: Journal of Electroanalytical Chemistry, Vol. 806, 01.12.2017, p. 123-129.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Label-free impedimetric glycosensor based on β-galactose-functionalized gold electrode for the determination of cholera toxin

AU - Jeong, Daeho

AU - Ahn, Kwang Soo

AU - Lee, Wonyong

PY - 2017/12/1

Y1 - 2017/12/1

N2 - A label-free impedimetric glycosensor has been developed for the direct detection of cholera toxin (CT). The glycosensor has been fabricated by forming the self-assembled monolayers of β-galactose derivatives containing poly-ethyleneglycol (PEG) spacer on gold electrode surfaces. The selective binding of CT to the β-galactose receptors on the fabricated glycosensor has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. gmonolayers on gold electrode surface has been determined to be ca. 2.53 × 1010 M− 1, indicating that the present glycosensor can selectively capture the target CT from sample solutions. Therefore, the present monosaccharide β-galactose derivative can be used as a receptor for CT as an alternative to CT-specific antibodies or pentasaccharide ganglioside GM1, most widely used in conventional CT assays. The present glycosensor can detect CT in the concentration range from 1.18 × 10− 12 M to 1.18 × 10− 9 M (r2 = 0.963) with a limit of detection (S/N = 3) down to 7.83 × 10− 13 M, which is much lower than those obtained with other detection methods. Since the present glycosensor-based CT assay does not require complex and time-consuming signal amplification steps generally employed in sandwich-type immunoassays, it enables the simple and rapid detection of CT.

AB - A label-free impedimetric glycosensor has been developed for the direct detection of cholera toxin (CT). The glycosensor has been fabricated by forming the self-assembled monolayers of β-galactose derivatives containing poly-ethyleneglycol (PEG) spacer on gold electrode surfaces. The selective binding of CT to the β-galactose receptors on the fabricated glycosensor has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. gmonolayers on gold electrode surface has been determined to be ca. 2.53 × 1010 M− 1, indicating that the present glycosensor can selectively capture the target CT from sample solutions. Therefore, the present monosaccharide β-galactose derivative can be used as a receptor for CT as an alternative to CT-specific antibodies or pentasaccharide ganglioside GM1, most widely used in conventional CT assays. The present glycosensor can detect CT in the concentration range from 1.18 × 10− 12 M to 1.18 × 10− 9 M (r2 = 0.963) with a limit of detection (S/N = 3) down to 7.83 × 10− 13 M, which is much lower than those obtained with other detection methods. Since the present glycosensor-based CT assay does not require complex and time-consuming signal amplification steps generally employed in sandwich-type immunoassays, it enables the simple and rapid detection of CT.

UR - http://www.scopus.com/inward/record.url?scp=85032971251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032971251&partnerID=8YFLogxK

U2 - 10.1016/j.jelechem.2017.10.064

DO - 10.1016/j.jelechem.2017.10.064

M3 - Article

VL - 806

SP - 123

EP - 129

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

SN - 0368-1874

ER -