A label-free impedimetric glycosensor has been developed for the direct detection of cholera toxin (CT). The glycosensor has been fabricated by forming the self-assembled monolayers of β-galactose derivatives containing poly-ethyleneglycol (PEG) spacer on gold electrode surfaces. The selective binding of CT to the β-galactose receptors on the fabricated glycosensor has been directly monitored by electrochemical impedance spectroscopy in the presence of 5.0 mM K3Fe(CN)6/K4Fe(CN)6 (1:1, v/v) redox couple. gmonolayers on gold electrode surface has been determined to be ca. 2.53 × 1010 M− 1, indicating that the present glycosensor can selectively capture the target CT from sample solutions. Therefore, the present monosaccharide β-galactose derivative can be used as a receptor for CT as an alternative to CT-specific antibodies or pentasaccharide ganglioside GM1, most widely used in conventional CT assays. The present glycosensor can detect CT in the concentration range from 1.18 × 10− 12 M to 1.18 × 10− 9 M (r2 = 0.963) with a limit of detection (S/N = 3) down to 7.83 × 10− 13 M, which is much lower than those obtained with other detection methods. Since the present glycosensor-based CT assay does not require complex and time-consuming signal amplification steps generally employed in sandwich-type immunoassays, it enables the simple and rapid detection of CT.
Bibliographical noteFunding Information:
Financial support for this work has been provided by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2015R1D1A1A01058396 ). This work was also supported by the Institute for Basic Science ( IBS-R026-D1 ).
© 2017 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Chemical Engineering(all)