Label-free isolation of circulating tumor cells (CTCs) from breast cancer patients using parallel multi-orifice flow fractionation (p-MOFF)

Kyung A. Hyun, Jung Hyun Lee, Seung Il Kim, Hyo Il Jung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Circulating tumor cells (CTCs), which are detached from primary cancer and circulate in peripheral blood, are known to cause the metastatic cancer. Therefore isolation of CTCs can serve as a powerful tool for cancer prognosis, diagnosis of minimal residual disease, assessment of tumor sensitivity to anticancer drugs, and personalization of anticancer therapy. In this paper, we introduce the parallel multi-orifice flow fractionation (p-MOFF) device which is connected by four single MOFF channel for the isolation of CTCs from whole blood of the metastatic breast cancer patients. For the experimentation of feasibility, we separated over 90% of human breast cancer cell line (MCF-7, MDA-MB-231 cells), and eliminate 90.80 % of WBCs from individual experiment at 600 μL/min inlet flow rate and 240 μL/min outlet flow rate. Based on this result, we have attempted to isolate CTCs from whole blood under the same conditions. Then we identify CTCs using immune staining method. Because our devices do not require any labeling processes (e.g. EpCAM antibody), heterogeneous CTCs can be isolated regardless of EpCAM expression.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages524-526
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Fractionation
Orifices
Labels
Tumors
Cells
Blood
Flow rate
Inlet flow
Antibodies
Labeling
Pharmaceutical Preparations
Experiments

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Hyun, K. A., Lee, J. H., Kim, S. I., & Jung, H. I. (2012). Label-free isolation of circulating tumor cells (CTCs) from breast cancer patients using parallel multi-orifice flow fractionation (p-MOFF). In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 524-526). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.
Hyun, Kyung A. ; Lee, Jung Hyun ; Kim, Seung Il ; Jung, Hyo Il. / Label-free isolation of circulating tumor cells (CTCs) from breast cancer patients using parallel multi-orifice flow fractionation (p-MOFF). Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 524-526 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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abstract = "Circulating tumor cells (CTCs), which are detached from primary cancer and circulate in peripheral blood, are known to cause the metastatic cancer. Therefore isolation of CTCs can serve as a powerful tool for cancer prognosis, diagnosis of minimal residual disease, assessment of tumor sensitivity to anticancer drugs, and personalization of anticancer therapy. In this paper, we introduce the parallel multi-orifice flow fractionation (p-MOFF) device which is connected by four single MOFF channel for the isolation of CTCs from whole blood of the metastatic breast cancer patients. For the experimentation of feasibility, we separated over 90{\%} of human breast cancer cell line (MCF-7, MDA-MB-231 cells), and eliminate 90.80 {\%} of WBCs from individual experiment at 600 μL/min inlet flow rate and 240 μL/min outlet flow rate. Based on this result, we have attempted to isolate CTCs from whole blood under the same conditions. Then we identify CTCs using immune staining method. Because our devices do not require any labeling processes (e.g. EpCAM antibody), heterogeneous CTCs can be isolated regardless of EpCAM expression.",
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Hyun, KA, Lee, JH, Kim, SI & Jung, HI 2012, Label-free isolation of circulating tumor cells (CTCs) from breast cancer patients using parallel multi-orifice flow fractionation (p-MOFF). in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 524-526, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.

Label-free isolation of circulating tumor cells (CTCs) from breast cancer patients using parallel multi-orifice flow fractionation (p-MOFF). / Hyun, Kyung A.; Lee, Jung Hyun; Kim, Seung Il; Jung, Hyo Il.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 524-526 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Hyun KA, Lee JH, Kim SI, Jung HI. Label-free isolation of circulating tumor cells (CTCs) from breast cancer patients using parallel multi-orifice flow fractionation (p-MOFF). In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 524-526. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).