Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

Ghi Yuun Kang, Ga Young Han, Ji Yoon Kang, Il Hoon Cho, Hyung Ho Park, Se Hwan Paek, Tae Song Kim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

This paper reports a method to improve the limit of the label-free detection of myoglobin using site-directed immobilization on the surface of self-actuating PZT cantilever sensor. The resonant frequency change of cantilever was measured electrically using piezoelectricity when antibody-antigen interaction occurred. Site-directed antibody was biotinylated after reducing the binding region to hinge region of randomly biotinylated antibody, which increased the number of effective binding sites. Investigating the effect of site-directed antibody on the cantilever sensor revealed that this protocol was able to enhance the performance of analytical sensitivity about 10 times with the modification of sensing layer. The experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

Original languageEnglish
Pages (from-to)332-338
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume117
Issue number2
DOIs
Publication statusPublished - 2006 Oct 12

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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