This paper reports a label-free detection of myoglobin using microfabricated self-actuating PZT cantilever. Electrical detection of antibody-antigen interaction on the surface was achieved through measuring the resonant frequency of cantilever. On Au surface of cantilever, biotinylated antibody bound to the streptavidin layer. We immobilized site-directed antibody through reducing the binding region to hinge region, which resolves the random arrangement of the biotinylated antibody and increases the number of binding complexes. This protocol enhanced the performance of analytical sensitivity about ten times, and the experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.
|Title of host publication||TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers|
|Number of pages||4|
|Publication status||Published - 2005|
|Event||13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of|
Duration: 2005 Jun 5 → 2005 Jun 9
|Name||Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05|
|Other||13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05|
|Country/Territory||Korea, Republic of|
|Period||05/6/5 → 05/6/9|
Bibliographical noteFunding Information:
This research has been supported by the Intelligent Microsystem Center (IMC; http://www.microsystem.re.kr ), which carries out one of the 21st century's Frontier R&D Projects sponsored by the Korea Ministry Of Commerce, Industry and Energy and a KIST GRANT Program sponsored by KIST.
All Science Journal Classification (ASJC) codes