Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

Ghi Yuun Kang, Ga Young Han, Ji Yoon Kang, Il Hoon Cho, Hyung Ho Park, Se Hwan Paek, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a label-free detection of myoglobin using microfabricated self-actuating PZT cantilever. Electrical detection of antibody-antigen interaction on the surface was achieved through measuring the resonant frequency of cantilever. On Au surface of cantilever, biotinylated antibody bound to the streptavidin layer. We immobilized site-directed antibody through reducing the binding region to hinge region, which resolves the random arrangement of the biotinylated antibody and increases the number of binding complexes. This protocol enhanced the performance of analytical sensitivity about ten times, and the experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

Original languageEnglish
Title of host publicationTRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages1812-1815
Number of pages4
Volume2
Publication statusPublished - 2005 Nov 9
Event13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of
Duration: 2005 Jun 52005 Jun 9

Other

Other13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
CountryKorea, Republic of
CitySeoul
Period05/6/505/6/9

Fingerprint

Antibodies
Labels
Assays
Proteins
Hinges
Antigens
Biosensors
Natural frequencies

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Kang, G. Y., Han, G. Y., Kang, J. Y., Cho, I. H., Park, H. H., Paek, S. H., & Kim, T. S. (2005). Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers (Vol. 2, pp. 1812-1815). [3E4.129]
Kang, Ghi Yuun ; Han, Ga Young ; Kang, Ji Yoon ; Cho, Il Hoon ; Park, Hyung Ho ; Paek, Se Hwan ; Kim, Tae Song. / Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever. TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. Vol. 2 2005. pp. 1812-1815
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title = "Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever",
abstract = "This paper reports a label-free detection of myoglobin using microfabricated self-actuating PZT cantilever. Electrical detection of antibody-antigen interaction on the surface was achieved through measuring the resonant frequency of cantilever. On Au surface of cantilever, biotinylated antibody bound to the streptavidin layer. We immobilized site-directed antibody through reducing the binding region to hinge region, which resolves the random arrangement of the biotinylated antibody and increases the number of binding complexes. This protocol enhanced the performance of analytical sensitivity about ten times, and the experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.",
author = "Kang, {Ghi Yuun} and Han, {Ga Young} and Kang, {Ji Yoon} and Cho, {Il Hoon} and Park, {Hyung Ho} and Paek, {Se Hwan} and Kim, {Tae Song}",
year = "2005",
month = "11",
day = "9",
language = "English",
isbn = "0780389948",
volume = "2",
pages = "1812--1815",
booktitle = "TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers",

}

Kang, GY, Han, GY, Kang, JY, Cho, IH, Park, HH, Paek, SH & Kim, TS 2005, Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever. in TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. vol. 2, 3E4.129, pp. 1812-1815, 13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05, Seoul, Korea, Republic of, 05/6/5.

Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever. / Kang, Ghi Yuun; Han, Ga Young; Kang, Ji Yoon; Cho, Il Hoon; Park, Hyung Ho; Paek, Se Hwan; Kim, Tae Song.

TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. Vol. 2 2005. p. 1812-1815 3E4.129.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

AU - Kang, Ghi Yuun

AU - Han, Ga Young

AU - Kang, Ji Yoon

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N2 - This paper reports a label-free detection of myoglobin using microfabricated self-actuating PZT cantilever. Electrical detection of antibody-antigen interaction on the surface was achieved through measuring the resonant frequency of cantilever. On Au surface of cantilever, biotinylated antibody bound to the streptavidin layer. We immobilized site-directed antibody through reducing the binding region to hinge region, which resolves the random arrangement of the biotinylated antibody and increases the number of binding complexes. This protocol enhanced the performance of analytical sensitivity about ten times, and the experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

AB - This paper reports a label-free detection of myoglobin using microfabricated self-actuating PZT cantilever. Electrical detection of antibody-antigen interaction on the surface was achieved through measuring the resonant frequency of cantilever. On Au surface of cantilever, biotinylated antibody bound to the streptavidin layer. We immobilized site-directed antibody through reducing the binding region to hinge region, which resolves the random arrangement of the biotinylated antibody and increases the number of binding complexes. This protocol enhanced the performance of analytical sensitivity about ten times, and the experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

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Kang GY, Han GY, Kang JY, Cho IH, Park HH, Paek SH et al. Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. Vol. 2. 2005. p. 1812-1815. 3E4.129