We study surface plasmon resonance microscopy (SPRM) for label-free quantification of cell-to-substrate separation. We have established a depth extraction model in which we compare a layered cell substrate model with resonance characteristics obtained by SPRM. We have applied the model to human aortic endothelial cell (HAEC) culture and determined the separation distance to be 40–60 nm. We have also investigated the precision of the SPRM model associated with the deviation in the model parameters, which is estimated to be 15 nm. The results can serve as the basis for more extensive cell-to-surface studies in a massive and automated way.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation (NRF) grants funded by the Korean Government (NRF- 2012R1A4A1029061 and 2015R1A2A1A10052826 ).
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Electrical and Electronic Engineering
- Atomic and Molecular Physics, and Optics
- Physical and Theoretical Chemistry