Label-free volumetric optical imaging of intact murine brains

Jian Ren, Heejin Choi, Kwanghun Chung, Brett E. Bouma

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


A central effort of today's neuroscience is to study the brain's 'wiring diagram'. The nervous system is believed to be a network of neurons interacting with each other through synaptic connection between axons and dendrites, therefore the neuronal connectivity map not only depicts the underlying anatomy, but also has important behavioral implications. Different approaches have been utilized to decipher neuronal circuits, including electron microscopy (EM) and light microscopy (LM). However, these approaches typically demand extensive sectioning and reconstruction for a brain sample. Recently, tissue clearing methods have enabled the investigation of a fully assembled biological system with greatly improved light penetration. Yet, most of these implementations, still require either genetic or exogenous contrast labeling for light microscopy. Here we demonstrate a high-speed approach, termed as Clearing Assisted Scattering Tomography (CAST), where intact brains can be imaged at optical resolution without labeling by leveraging tissue clearing and the scattering contrast of optical frequency domain imaging (OFDI).

Original languageEnglish
Article number46306
JournalScientific reports
Publication statusPublished - 2017 Apr 12

Bibliographical note

Funding Information:
The authors acknowledge partial funding support from the National Institutes of Health, grant P41EB015903.

Publisher Copyright:
© The Author(s) 2017.

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'Label-free volumetric optical imaging of intact murine brains'. Together they form a unique fingerprint.

Cite this