Lack of effect of dexamethasone on growth of Orientia Tsutsugamushi Gilliam in mouse L929 cells

Chang Oh Kim, Ae Jung Huh, Joon Sup Yeom, Kkot Sil Lee, Bum Sik Chin, Sang Hoon Han, Su Jin Jeong, Jun Yong Choi, Young Goo Song, June Myung Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease. Materials and Methods: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 101 and 107 pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone. Results: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fuorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 101 and 107 pg/mL showed no influence on the growth of Orientia tsutsugamushi. Conclusion: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

Original languageEnglish
Pages (from-to)624-629
Number of pages6
JournalYonsei medical journal
Volume52
Issue number4
DOIs
Publication statusPublished - 2011 Jul 1

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Orientia tsutsugamushi
Dexamethasone
Adrenal Cortex Hormones
Growth
Scrub Typhus
Immunosuppressive Agents
Flow Cytometry
Anti-Inflammatory Agents
Anti-Bacterial Agents
Mortality

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Kim, C. O., Huh, A. J., Yeom, J. S., Lee, K. S., Chin, B. S., Han, S. H., ... Kim, J. M. (2011). Lack of effect of dexamethasone on growth of Orientia Tsutsugamushi Gilliam in mouse L929 cells. Yonsei medical journal, 52(4), 624-629. https://doi.org/10.3349/ymj.2011.52.4.624
Kim, Chang Oh ; Huh, Ae Jung ; Yeom, Joon Sup ; Lee, Kkot Sil ; Chin, Bum Sik ; Han, Sang Hoon ; Jeong, Su Jin ; Choi, Jun Yong ; Song, Young Goo ; Kim, June Myung. / Lack of effect of dexamethasone on growth of Orientia Tsutsugamushi Gilliam in mouse L929 cells. In: Yonsei medical journal. 2011 ; Vol. 52, No. 4. pp. 624-629.
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Kim, CO, Huh, AJ, Yeom, JS, Lee, KS, Chin, BS, Han, SH, Jeong, SJ, Choi, JY, Song, YG & Kim, JM 2011, 'Lack of effect of dexamethasone on growth of Orientia Tsutsugamushi Gilliam in mouse L929 cells', Yonsei medical journal, vol. 52, no. 4, pp. 624-629. https://doi.org/10.3349/ymj.2011.52.4.624

Lack of effect of dexamethasone on growth of Orientia Tsutsugamushi Gilliam in mouse L929 cells. / Kim, Chang Oh; Huh, Ae Jung; Yeom, Joon Sup; Lee, Kkot Sil; Chin, Bum Sik; Han, Sang Hoon; Jeong, Su Jin; Choi, Jun Yong; Song, Young Goo; Kim, June Myung.

In: Yonsei medical journal, Vol. 52, No. 4, 01.07.2011, p. 624-629.

Research output: Contribution to journalArticle

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T1 - Lack of effect of dexamethasone on growth of Orientia Tsutsugamushi Gilliam in mouse L929 cells

AU - Kim, Chang Oh

AU - Huh, Ae Jung

AU - Yeom, Joon Sup

AU - Lee, Kkot Sil

AU - Chin, Bum Sik

AU - Han, Sang Hoon

AU - Jeong, Su Jin

AU - Choi, Jun Yong

AU - Song, Young Goo

AU - Kim, June Myung

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Y1 - 2011/7/1

N2 - Purpose: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease. Materials and Methods: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 101 and 107 pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone. Results: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fuorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 101 and 107 pg/mL showed no influence on the growth of Orientia tsutsugamushi. Conclusion: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

AB - Purpose: Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease. Materials and Methods: L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 101 and 107 pg/mL. Cultures were incubated at 35°C and processed for flow cytometry on the 6th day after addition of dexamethasone. Results: Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fuorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 101 and 107 pg/mL showed no influence on the growth of Orientia tsutsugamushi. Conclusion: Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.

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