Lactobacillus acidophilus Antimicrobial Peptide Is Antagonistic to Aeromonas hydrophila

Nahid Akter; Roshada Hashim; Huy Quang Pham; Seung Dae Choi; Dong Woo Lee; Jae Ho Shin; Kammara Rajagopal

doi:10.3389/fmicb.2020.570851

Lactobacillus acidophilus Antimicrobial Peptide Is Antagonistic to Aeromonas hydrophila

Nahid Akter, Roshada Hashim, Huy Quang Pham, Seung Dae Choi, Dong Woo Lee, Jae Ho Shin, Kammara Rajagopal

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We identified an antimicrobial peptide (AMP) from Lactobacillus acidophilus that was antagonistic to Aeromonas hydrophila. In vitro studies such as well-diffusion and field trials revealed that the AMP was active against A. hydrophila. The field trials of AMP using A. hydrophila-infected Channa striatus with a mannone oligosaccharide (MOS) prebiotic, A. hydrophila antigens, A. hydrophila-infected fish serum, L. acidophilus, and Lactobacillus cell free-supernatant (LABS-CFS) on an indicator organism further revealed that the antimicrobial agent could protect C. striatus. Other than the AMP, none of the above were able to eliminate the infectious agent A. hydrophila, and were only able to delay the death rate for 3–4 days. Thus, we conclude that the AMP is antagonistic to A. hydrophila and may be used for treatment of A. hydrophila infections. Subsequent L. acidophilus whole-genome sequence analyses enabled an understanding of the (probable) gene arrangement and its location on the chromosome. This information may be useful in the generation of recombinant peptides to produce larger quantities for treatment.

Original language	English
Article number	570851
Journal	Frontiers in Microbiology
Volume	11
DOIs	https://doi.org/10.3389/fmicb.2020.570851
Publication status	Published - 2020 Oct 9

Bibliographical note

Funding Information:
We would like to acknowledge CSIR-CFTRI, CSIR-IMT, Third World Academy of Sciences (TWAS) – University of Sains, Malaysia (TWAS – USM) for financial assistance, to KR and the KyungPook National University (KNU)-fellowship to Phaum.

Funding Information:
We would like to acknowledge CSIR-CFTRI, CSIR-IMT, Third World Academy of Sciences (TWAS) ? University of Sains, Malaysia (TWAS ? USM) for financial assistance, to KR and the KyungPook National University (KNU)-fellowship to Phaum. At-last, but not the least we thank Anagha N. a research student for her enthusiastic contributions. Funding. The research was financially, supported by the Strategic Initiative for Microbiomes in Agriculture and Food (Grant Number 918010-4), Ministry of Agriculture, Food and Rural Affairs, South Korea. Funding from USM, Malaysia and TWAS is highly appreciated.

Funding Information:
The research was financially, supported by the Strategic Initiative for Microbiomes in Agriculture and Food (Grant

Publisher Copyright:
© Copyright © 2020 Akter, Hashim, Pham, Choi, Lee, Shin and Rajagopal.

All Science Journal Classification (ASJC) codes

Microbiology
Microbiology (medical)

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10.3389/fmicb.2020.570851

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title = "Lactobacillus acidophilus Antimicrobial Peptide Is Antagonistic to Aeromonas hydrophila",

abstract = "We identified an antimicrobial peptide (AMP) from Lactobacillus acidophilus that was antagonistic to Aeromonas hydrophila. In vitro studies such as well-diffusion and field trials revealed that the AMP was active against A. hydrophila. The field trials of AMP using A. hydrophila-infected Channa striatus with a mannone oligosaccharide (MOS) prebiotic, A. hydrophila antigens, A. hydrophila-infected fish serum, L. acidophilus, and Lactobacillus cell free-supernatant (LABS-CFS) on an indicator organism further revealed that the antimicrobial agent could protect C. striatus. Other than the AMP, none of the above were able to eliminate the infectious agent A. hydrophila, and were only able to delay the death rate for 3–4 days. Thus, we conclude that the AMP is antagonistic to A. hydrophila and may be used for treatment of A. hydrophila infections. Subsequent L. acidophilus whole-genome sequence analyses enabled an understanding of the (probable) gene arrangement and its location on the chromosome. This information may be useful in the generation of recombinant peptides to produce larger quantities for treatment.",

author = "Nahid Akter and Roshada Hashim and Pham, {Huy Quang} and Choi, {Seung Dae} and Lee, {Dong Woo} and Shin, {Jae Ho} and Kammara Rajagopal",

note = "Funding Information: We would like to acknowledge CSIR-CFTRI, CSIR-IMT, Third World Academy of Sciences (TWAS) – University of Sains, Malaysia (TWAS – USM) for financial assistance, to KR and the KyungPook National University (KNU)-fellowship to Phaum. Funding Information: We would like to acknowledge CSIR-CFTRI, CSIR-IMT, Third World Academy of Sciences (TWAS) ? University of Sains, Malaysia (TWAS ? USM) for financial assistance, to KR and the KyungPook National University (KNU)-fellowship to Phaum. At-last, but not the least we thank Anagha N. a research student for her enthusiastic contributions. Funding. The research was financially, supported by the Strategic Initiative for Microbiomes in Agriculture and Food (Grant Number 918010-4), Ministry of Agriculture, Food and Rural Affairs, South Korea. Funding from USM, Malaysia and TWAS is highly appreciated. Funding Information: The research was financially, supported by the Strategic Initiative for Microbiomes in Agriculture and Food (Grant Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Akter, Hashim, Pham, Choi, Lee, Shin and Rajagopal.",

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AU - Choi, Seung Dae

AU - Lee, Dong Woo

AU - Shin, Jae Ho

AU - Rajagopal, Kammara

N1 - Funding Information: We would like to acknowledge CSIR-CFTRI, CSIR-IMT, Third World Academy of Sciences (TWAS) – University of Sains, Malaysia (TWAS – USM) for financial assistance, to KR and the KyungPook National University (KNU)-fellowship to Phaum. Funding Information: We would like to acknowledge CSIR-CFTRI, CSIR-IMT, Third World Academy of Sciences (TWAS) ? University of Sains, Malaysia (TWAS ? USM) for financial assistance, to KR and the KyungPook National University (KNU)-fellowship to Phaum. At-last, but not the least we thank Anagha N. a research student for her enthusiastic contributions. Funding. The research was financially, supported by the Strategic Initiative for Microbiomes in Agriculture and Food (Grant Number 918010-4), Ministry of Agriculture, Food and Rural Affairs, South Korea. Funding from USM, Malaysia and TWAS is highly appreciated. Funding Information: The research was financially, supported by the Strategic Initiative for Microbiomes in Agriculture and Food (Grant Publisher Copyright: © Copyright © 2020 Akter, Hashim, Pham, Choi, Lee, Shin and Rajagopal.

PY - 2020/10/9

Y1 - 2020/10/9

N2 - We identified an antimicrobial peptide (AMP) from Lactobacillus acidophilus that was antagonistic to Aeromonas hydrophila. In vitro studies such as well-diffusion and field trials revealed that the AMP was active against A. hydrophila. The field trials of AMP using A. hydrophila-infected Channa striatus with a mannone oligosaccharide (MOS) prebiotic, A. hydrophila antigens, A. hydrophila-infected fish serum, L. acidophilus, and Lactobacillus cell free-supernatant (LABS-CFS) on an indicator organism further revealed that the antimicrobial agent could protect C. striatus. Other than the AMP, none of the above were able to eliminate the infectious agent A. hydrophila, and were only able to delay the death rate for 3–4 days. Thus, we conclude that the AMP is antagonistic to A. hydrophila and may be used for treatment of A. hydrophila infections. Subsequent L. acidophilus whole-genome sequence analyses enabled an understanding of the (probable) gene arrangement and its location on the chromosome. This information may be useful in the generation of recombinant peptides to produce larger quantities for treatment.

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ER -

Lactobacillus acidophilus Antimicrobial Peptide Is Antagonistic to Aeromonas hydrophila

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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