Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling

Jeong Won Hwang; Young Mi Baek; Kyeong Eun Yang; Hwa Seung Yoo; Chong Kwan Cho; Yeon Weol Lee; Junsoo Park; Chi Yong Eom; Zee Won Lee; Jong Soon Choi; Ik Soon Jang

doi:10.1177/1534735412442380

Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling

Jeong Won Hwang, Young Mi Baek, Kyeong Eun Yang, Hwa Seung Yoo, Chong Kwan Cho, Yeon Weol Lee, Junsoo Park, Chi Yong Eom, Zee Won Lee, Jong Soon Choi, Ik Soon Jang

Division of Bioscience and Biotechnology

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30 Citations (Scopus)

Abstract

Lactobacillus casei extract (LBX) has been reported to prevent gastric cancer, but the underlying mechanism remains unclear. The proliferation and cell death of gastric cancer KATO3 cells were examined after treatment with LBX for various times and at various doses. LBX inhibited the growth of gastric cancer cells and induced apoptosis by inactivating NF-κB promoter activity. Apoptosis induced by LBX, however, is not directly associated with the intrinsic mitochondrial pathway. Immunoblot analysis revealed that LBX decreased the expressions of NF-κB and IκB. The reduced NF-κB levels led to the decreased phosphorylation of mTOR signaling components, such as PI3K, Akt, and ^p70S6 kinase. These results showed for the first time that LBX induced apoptosis in gastric cancer cells by inhibiting NF-κB and mTOR-mediated signaling.

Original language	English
Pages (from-to)	165-173
Number of pages	9
Journal	Integrative Cancer Therapies
Volume	12
Issue number	2
DOIs	https://doi.org/10.1177/1534735412442380
Publication status	Published - 2013 Mar

Bibliographical note

Funding Information:
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by a Korea Basic Science Institute NAP grant (T3178B) to J. S. Choi and by the Mid-career Researcher Program through an NRF grant funded by the MEST (No. No RO1-2008-000-20253-0).

All Science Journal Classification (ASJC) codes

Oncology
Complementary and alternative medicine

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Hwang, Jeong Won ; Baek, Young Mi ; Yang, Kyeong Eun ; Yoo, Hwa Seung ; Cho, Chong Kwan ; Lee, Yeon Weol ; Park, Junsoo ; Eom, Chi Yong ; Lee, Zee Won ; Choi, Jong Soon ; Jang, Ik Soon. / Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling. In: Integrative Cancer Therapies. 2013 ; Vol. 12, No. 2. pp. 165-173.

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title = "Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling",

abstract = "Lactobacillus casei extract (LBX) has been reported to prevent gastric cancer, but the underlying mechanism remains unclear. The proliferation and cell death of gastric cancer KATO3 cells were examined after treatment with LBX for various times and at various doses. LBX inhibited the growth of gastric cancer cells and induced apoptosis by inactivating NF-κB promoter activity. Apoptosis induced by LBX, however, is not directly associated with the intrinsic mitochondrial pathway. Immunoblot analysis revealed that LBX decreased the expressions of NF-κB and IκB. The reduced NF-κB levels led to the decreased phosphorylation of mTOR signaling components, such as PI3K, Akt, and p70S6 kinase. These results showed for the first time that LBX induced apoptosis in gastric cancer cells by inhibiting NF-κB and mTOR-mediated signaling.",

author = "Hwang, {Jeong Won} and Baek, {Young Mi} and Yang, {Kyeong Eun} and Yoo, {Hwa Seung} and Cho, {Chong Kwan} and Lee, {Yeon Weol} and Junsoo Park and Eom, {Chi Yong} and Lee, {Zee Won} and Choi, {Jong Soon} and Jang, {Ik Soon}",

note = "Funding Information: The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by a Korea Basic Science Institute NAP grant (T3178B) to J. S. Choi and by the Mid-career Researcher Program through an NRF grant funded by the MEST (No. No RO1-2008-000-20253-0).",

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doi = "10.1177/1534735412442380",

language = "English",

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Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling. / Hwang, Jeong Won; Baek, Young Mi; Yang, Kyeong Eun; Yoo, Hwa Seung; Cho, Chong Kwan; Lee, Yeon Weol; Park, Junsoo; Eom, Chi Yong; Lee, Zee Won; Choi, Jong Soon; Jang, Ik Soon.

In: Integrative Cancer Therapies, Vol. 12, No. 2, 03.2013, p. 165-173.

Research output: Contribution to journal › Article › peer-review

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AU - Lee, Zee Won

AU - Choi, Jong Soon

AU - Jang, Ik Soon

N1 - Funding Information: The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by a Korea Basic Science Institute NAP grant (T3178B) to J. S. Choi and by the Mid-career Researcher Program through an NRF grant funded by the MEST (No. No RO1-2008-000-20253-0).

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AB - Lactobacillus casei extract (LBX) has been reported to prevent gastric cancer, but the underlying mechanism remains unclear. The proliferation and cell death of gastric cancer KATO3 cells were examined after treatment with LBX for various times and at various doses. LBX inhibited the growth of gastric cancer cells and induced apoptosis by inactivating NF-κB promoter activity. Apoptosis induced by LBX, however, is not directly associated with the intrinsic mitochondrial pathway. Immunoblot analysis revealed that LBX decreased the expressions of NF-κB and IκB. The reduced NF-κB levels led to the decreased phosphorylation of mTOR signaling components, such as PI3K, Akt, and p70S6 kinase. These results showed for the first time that LBX induced apoptosis in gastric cancer cells by inhibiting NF-κB and mTOR-mediated signaling.

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Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling

Abstract

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