Laminin-332-rich tumor microenvironment for tumor invasion in the interface zone of breast cancer

Baek Gil Kim, Hee Jung An, Suki Kang, Yoon Pyo Choi, Ming Qing Gao, Haengran Park, Nam Hoon Cho

Research output: Contribution to journalArticle

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Abstract

Dense fibrosis, which is caused by desmoplastic reaction, is usually found in invasive ductal carcinoma and may represent the alteration of the tumor microenvironment preceding tumor invasion. Thus, the dense fibrotic zone around invasive ductal carcinoma can be considered to be the actual tissue site of tumor microenvironment, where the precedent alterations for tumor invasion occur. To characterize the dense fibrotic zone, we classified invasive ductal carcinoma tissue into a tumor zone, a normal zone, and the novel interface zone (IZ), which shows dense fibrosis. The postulated IZ is a 5-mm-wide belt that circles the tumor margin and overlaps with normal tissue. Of the extracellular matrix components, laminin-332 was specifically overexpressed in the IZ. Events that appear to be similar to the epithelial-mesenchymal transition, a novel source of myofibroblast formation from epithelial cells, were observed in the IZ, according to the following characteristics: overexpression of matrix metalloproteinase 3, membrane type 1-matrix metalloproteinase, snail, and zinc finger E-box-binding homeobox 1, and the gain of N-cadherin expression, as well as the down-regulation of miR200c. The myofibroblasts isolated from the IZ, which were designated interface zone-fibroblast, displayed laminin-332 and membrane type 1-matrix metalloproteinase overexpression, in contrast with both cancer-associated fibroblasts and normal breast fibroblasts. Taken together, our results suggest that the IZ, which shows dense fibrosis, may provide a specialized microenvironment for guiding tumor invasion: the fibrosis caused by laminin-332 overexpressing myofibroblast formation (interface zone-fibroblast) via epithelialmesenchymal transition.

Original languageEnglish
Pages (from-to)373-381
Number of pages9
JournalAmerican Journal of Pathology
Volume178
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Fingerprint

Tumor Microenvironment
Ductal Carcinoma
Myofibroblasts
Fibrosis
Breast Neoplasms
Matrix Metalloproteinase 14
Fibroblasts
Neoplasms
Matrix Metalloproteinase 3
Epithelial-Mesenchymal Transition
Snails
Cadherins
Extracellular Matrix
Breast
Down-Regulation
Epithelial Cells
kalinin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Kim, Baek Gil ; An, Hee Jung ; Kang, Suki ; Choi, Yoon Pyo ; Gao, Ming Qing ; Park, Haengran ; Cho, Nam Hoon. / Laminin-332-rich tumor microenvironment for tumor invasion in the interface zone of breast cancer. In: American Journal of Pathology. 2011 ; Vol. 178, No. 1. pp. 373-381.
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abstract = "Dense fibrosis, which is caused by desmoplastic reaction, is usually found in invasive ductal carcinoma and may represent the alteration of the tumor microenvironment preceding tumor invasion. Thus, the dense fibrotic zone around invasive ductal carcinoma can be considered to be the actual tissue site of tumor microenvironment, where the precedent alterations for tumor invasion occur. To characterize the dense fibrotic zone, we classified invasive ductal carcinoma tissue into a tumor zone, a normal zone, and the novel interface zone (IZ), which shows dense fibrosis. The postulated IZ is a 5-mm-wide belt that circles the tumor margin and overlaps with normal tissue. Of the extracellular matrix components, laminin-332 was specifically overexpressed in the IZ. Events that appear to be similar to the epithelial-mesenchymal transition, a novel source of myofibroblast formation from epithelial cells, were observed in the IZ, according to the following characteristics: overexpression of matrix metalloproteinase 3, membrane type 1-matrix metalloproteinase, snail, and zinc finger E-box-binding homeobox 1, and the gain of N-cadherin expression, as well as the down-regulation of miR200c. The myofibroblasts isolated from the IZ, which were designated interface zone-fibroblast, displayed laminin-332 and membrane type 1-matrix metalloproteinase overexpression, in contrast with both cancer-associated fibroblasts and normal breast fibroblasts. Taken together, our results suggest that the IZ, which shows dense fibrosis, may provide a specialized microenvironment for guiding tumor invasion: the fibrosis caused by laminin-332 overexpressing myofibroblast formation (interface zone-fibroblast) via epithelialmesenchymal transition.",
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Laminin-332-rich tumor microenvironment for tumor invasion in the interface zone of breast cancer. / Kim, Baek Gil; An, Hee Jung; Kang, Suki; Choi, Yoon Pyo; Gao, Ming Qing; Park, Haengran; Cho, Nam Hoon.

In: American Journal of Pathology, Vol. 178, No. 1, 01.2011, p. 373-381.

Research output: Contribution to journalArticle

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