Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

Mi Hyun Lee, Bohye Kim, Sang Kee Song, Jung Ok Heo, Nan Ie Yu, Shin Ae Lee, Miran Kim, Dong Gwan Kim, Sung Oh Sohn, Chae Eun Lim, Kwang Suk Chang, Myeong Min Lee, Jun Lim

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.

Original languageEnglish
Pages (from-to)659-670
Number of pages12
JournalPlant Molecular Biology
Volume67
Issue number6
DOIs
Publication statusPublished - 2008 Aug 1

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Arabidopsis
Arabidopsis thaliana
protein-protein interactions
transcription factors
Genes
two hybrid system techniques
Proteins
genes
proteins
pseudogenes
plant development
growth and development
reverse transcriptase polymerase chain reaction
promoter regions
plant growth
phenotype
mutants
genome
Reverse Genetics
Two-Hybrid System Techniques

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Lee, M. H., Kim, B., Song, S. K., Heo, J. O., Yu, N. I., Lee, S. A., ... Lim, J. (2008). Large-scale analysis of the GRAS gene family in Arabidopsis thaliana. Plant Molecular Biology, 67(6), 659-670. https://doi.org/10.1007/s11103-008-9345-1
Lee, Mi Hyun ; Kim, Bohye ; Song, Sang Kee ; Heo, Jung Ok ; Yu, Nan Ie ; Lee, Shin Ae ; Kim, Miran ; Kim, Dong Gwan ; Sohn, Sung Oh ; Lim, Chae Eun ; Chang, Kwang Suk ; Lee, Myeong Min ; Lim, Jun. / Large-scale analysis of the GRAS gene family in Arabidopsis thaliana. In: Plant Molecular Biology. 2008 ; Vol. 67, No. 6. pp. 659-670.
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Lee, MH, Kim, B, Song, SK, Heo, JO, Yu, NI, Lee, SA, Kim, M, Kim, DG, Sohn, SO, Lim, CE, Chang, KS, Lee, MM & Lim, J 2008, 'Large-scale analysis of the GRAS gene family in Arabidopsis thaliana', Plant Molecular Biology, vol. 67, no. 6, pp. 659-670. https://doi.org/10.1007/s11103-008-9345-1

Large-scale analysis of the GRAS gene family in Arabidopsis thaliana. / Lee, Mi Hyun; Kim, Bohye; Song, Sang Kee; Heo, Jung Ok; Yu, Nan Ie; Lee, Shin Ae; Kim, Miran; Kim, Dong Gwan; Sohn, Sung Oh; Lim, Chae Eun; Chang, Kwang Suk; Lee, Myeong Min; Lim, Jun.

In: Plant Molecular Biology, Vol. 67, No. 6, 01.08.2008, p. 659-670.

Research output: Contribution to journalArticle

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T1 - Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

AU - Lee, Mi Hyun

AU - Kim, Bohye

AU - Song, Sang Kee

AU - Heo, Jung Ok

AU - Yu, Nan Ie

AU - Lee, Shin Ae

AU - Kim, Miran

AU - Kim, Dong Gwan

AU - Sohn, Sung Oh

AU - Lim, Chae Eun

AU - Chang, Kwang Suk

AU - Lee, Myeong Min

AU - Lim, Jun

PY - 2008/8/1

Y1 - 2008/8/1

N2 - GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.

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