LCCL peptide cleavage after noise exposure exacerbates hearing loss and is associated with the monocyte infiltration in the cochlea

Seong Hoon Bae, Jee Eun Yoo, Ji Won Hong, Haeng Ran Park, Byunghwa Noh, Hyoyeol Kim, Minjin Kang, Young Min Hyun, Heon Yung Gee, Jae Young Choi, Jinsei Jung

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Acoustic trauma induces an inflammatory response in the cochlea, resulting in debilitating hearing function. Clinically, amelioration of inflammation substantially prevents noise-induced hearing loss. The Limulus factor C, Cochlin, and Lgl1 (LCCL) peptide plays an important role in innate immunity during bacteria-induced inflammation in the cochlea. We aimed to investigate the LCCL-induced innate immune response to noise exposure and its impact on hearing function. Methods: We used Coch (encodes cochlin harboring LCCL peptide) knock-out and p.G88E knock-in mice to compare the immune responses before and after noise exposure. We explored their hearing function and hair cell degeneration. Moreover, we investigated distinct characteristics of immune responses upon noise exposure using flow cytometry and RNA sequencing. Results: One day after noise exposure, the LCCL peptide cleaved from cochlin increased over time in the perilymph space. Both Coch−/− and CochG88E/G88E mutant mice revealed more preserved hearing following acoustic trauma compared to wild-type mice. The outer hair cells were more preserved in Coch−/− than in wild-type mice upon noise exposure. The RNA sequencing data demonstrated significantly upregulated cell migration gene ontology in wild-type mice than in Coch−/− mice following noise exposure, indicating that the infiltration of immune cells was dependent on cochlin. Notably, infiltrated monocytes from blood (C11b+/Ly6G/Ly6C+) were remarkably higher in wild-type mice than in Coch−/− mice at 1 day after noise exposure. Conclusions: Noise-induced hearing loss was attributed to over-stimulated cochlin, and led to the cleavage and secretion of LCCL peptide in the cochlea. The LCCL peptide recruited more monocytes from the blood vessels upon noise stimulation, thus highlighting a novel therapeutic target for noise-induced hearing loss.

Original languageEnglish
Article number108378
JournalHearing Research
Volume412
DOIs
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
This research was supported by the Team Science Award of Yonsei University College of Medicine (6-2021-0002 to JJ) and the Basic Research Program of the National Research Foundation of Korea (NRF), and funded by the Ministry of Science, ICT & Future Planning (2019R1A2C1084033 to JJ) and the Ministry of Education (NRF-2020R1I1A1A01067241 to S.H.B).

Funding Information:
This research was supported by the Team Science Award of Yonsei University College of Medicine (6-2021-0002 to JJ) and the Basic Research Program of the National Research Foundation of Korea (NRF), and funded by the Ministry of Science, ICT & Future Planning (2019R1A2C1084033 to JJ) and the Ministry of Education (NRF-2020R1I1A1A01067241 to S.H.B).

Publisher Copyright:
© 2021

All Science Journal Classification (ASJC) codes

  • Sensory Systems

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