Leptin regulates the pro-inflammatory response in human epidermal keratinocytes

Moonyoung Lee, Eunyoung Lee, Sun Hee Jin, Sungjin Ahn, Sae On Kim, Jungmin Kim, Dalwoong Choi, Kyung Min Lim, Seung Taek Lee, Minsoo Noh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The role of leptin in cutaneous wound healing process has been suggested in genetically obese mouse studies. However, the molecular and cellular effects of leptin on human epidermal keratinocytes are still unclear. In this study, the whole-genome-scale microarray analysis was performed to elucidate the effect of leptin on epidermal keratinocyte functions. In the leptin-treated normal human keratinocytes (NHKs), we identified the 151 upregulated and 53 downregulated differentially expressed genes (DEGs). The gene ontology (GO) enrichment analysis with the leptin-induced DEGs suggests that leptin regulates NHKs to promote pro-inflammatory responses, extracellular matrix organization, and angiogenesis. Among the DEGs, the protein expression of IL-8, MMP-1, fibronectin, and S100A7, which play roles in which is important in the regulation of cutaneous inflammation, was confirmed in the leptin-treated NHKs. The upregulation of the leptin-induced proteins is mainly regulated by the STAT3 signaling pathway in NHKs. Among the downregulated DEGs, the protein expression of nucleosome assembly-associated centromere protein A (CENPA) and CENPM was confirmed in the leptin-treated NHKs. However, the expression of CENPA and CENPM was not coupled with those of other chromosome passenger complex like Aurora A kinase, INCENP, and survivin. In cell growth kinetics analysis, leptin had no significant effect on the cell growth curves of NHKs in the normal growth factor-enriched condition. Therefore, leptin-dependent downregulation of CENPA and CENPM in NHKs may not be directly associated with mitotic regulation during inflammation.

Original languageEnglish
Pages (from-to)351-362
Number of pages12
JournalArchives of Dermatological Research
Volume310
Issue number4
DOIs
Publication statusPublished - 2018 May 1

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Leptin
Keratinocytes
Down-Regulation
Aurora Kinase A
Genes
Inflammation
Obese Mice
Skin
Gene Ontology
Proteins
Nucleosomes
Microarray Analysis
Growth
Matrix Metalloproteinases
Interleukin-8
Fibronectins
Wound Healing
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Lee, Moonyoung ; Lee, Eunyoung ; Jin, Sun Hee ; Ahn, Sungjin ; Kim, Sae On ; Kim, Jungmin ; Choi, Dalwoong ; Lim, Kyung Min ; Lee, Seung Taek ; Noh, Minsoo. / Leptin regulates the pro-inflammatory response in human epidermal keratinocytes. In: Archives of Dermatological Research. 2018 ; Vol. 310, No. 4. pp. 351-362.
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abstract = "The role of leptin in cutaneous wound healing process has been suggested in genetically obese mouse studies. However, the molecular and cellular effects of leptin on human epidermal keratinocytes are still unclear. In this study, the whole-genome-scale microarray analysis was performed to elucidate the effect of leptin on epidermal keratinocyte functions. In the leptin-treated normal human keratinocytes (NHKs), we identified the 151 upregulated and 53 downregulated differentially expressed genes (DEGs). The gene ontology (GO) enrichment analysis with the leptin-induced DEGs suggests that leptin regulates NHKs to promote pro-inflammatory responses, extracellular matrix organization, and angiogenesis. Among the DEGs, the protein expression of IL-8, MMP-1, fibronectin, and S100A7, which play roles in which is important in the regulation of cutaneous inflammation, was confirmed in the leptin-treated NHKs. The upregulation of the leptin-induced proteins is mainly regulated by the STAT3 signaling pathway in NHKs. Among the downregulated DEGs, the protein expression of nucleosome assembly-associated centromere protein A (CENPA) and CENPM was confirmed in the leptin-treated NHKs. However, the expression of CENPA and CENPM was not coupled with those of other chromosome passenger complex like Aurora A kinase, INCENP, and survivin. In cell growth kinetics analysis, leptin had no significant effect on the cell growth curves of NHKs in the normal growth factor-enriched condition. Therefore, leptin-dependent downregulation of CENPA and CENPM in NHKs may not be directly associated with mitotic regulation during inflammation.",
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Lee, M, Lee, E, Jin, SH, Ahn, S, Kim, SO, Kim, J, Choi, D, Lim, KM, Lee, ST & Noh, M 2018, 'Leptin regulates the pro-inflammatory response in human epidermal keratinocytes', Archives of Dermatological Research, vol. 310, no. 4, pp. 351-362. https://doi.org/10.1007/s00403-018-1821-0

Leptin regulates the pro-inflammatory response in human epidermal keratinocytes. / Lee, Moonyoung; Lee, Eunyoung; Jin, Sun Hee; Ahn, Sungjin; Kim, Sae On; Kim, Jungmin; Choi, Dalwoong; Lim, Kyung Min; Lee, Seung Taek; Noh, Minsoo.

In: Archives of Dermatological Research, Vol. 310, No. 4, 01.05.2018, p. 351-362.

Research output: Contribution to journalArticle

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T1 - Leptin regulates the pro-inflammatory response in human epidermal keratinocytes

AU - Lee, Moonyoung

AU - Lee, Eunyoung

AU - Jin, Sun Hee

AU - Ahn, Sungjin

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AU - Lee, Seung Taek

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