Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells

Jesang Ko, In Sik Kim, Sung Wuk Jang, Young Han Lee, Soon Young Shin, Do Sik Min, Doe Sun Na

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalFEBS Letters
Volume515
Issue number1-3
DOIs
Publication statusPublished - 2002 Mar 27

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells'. Together they form a unique fingerprint.

  • Cite this

    Ko, J., Kim, I. S., Jang, S. W., Lee, Y. H., Shin, S. Y., Min, D. S., & Na, D. S. (2002). Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. FEBS Letters, 515(1-3), 159-164. https://doi.org/10.1016/S0014-5793(02)02465-1