Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells

Jesang Ko, In Sik Kim, Sung Wuk Jang, Young Han Lee, Soon Young Shin, Do Sik Min, Doe Sun Na

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalFEBS Letters
Volume515
Issue number1-3
DOIs
Publication statusPublished - 2002 Mar 27

Bibliographical note

Funding Information:
This work was supported by the research fund to Immunomodulation Research Center, University of Ulsan from Korea Science and Engineering Foundation (KOSEF).

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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