Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells

Jesang Ko, In Sik Kim, Sung Wuk Jang, Young Han Lee, Soon Young Shin, Do Sik Min, Doe Sun Na

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalFEBS Letters
Volume515
Issue number1-3
DOIs
Publication statusPublished - 2002 Mar 27

Fingerprint

Type C Phospholipases
Chemotaxis
Protein Kinase C
Complement Factor B
Protein C
Proteins
CC Chemokines
Chemotactic Factors
Dactinomycin
Osteosarcoma
Cycloheximide
Human Activities
Lymphocytes
Monocytes
Neutrophils
Cells
DNA
Molecules
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ko, J., Kim, I. S., Jang, S. W., Lee, Y. H., Shin, S. Y., Min, D. S., & Na, D. S. (2002). Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. FEBS Letters, 515(1-3), 159-164. https://doi.org/10.1016/S0014-5793(02)02465-1
Ko, Jesang ; Kim, In Sik ; Jang, Sung Wuk ; Lee, Young Han ; Shin, Soon Young ; Min, Do Sik ; Na, Doe Sun. / Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. In: FEBS Letters. 2002 ; Vol. 515, No. 1-3. pp. 159-164.
@article{bd2183e7b6e74e28b13bf2e2277ea72c,
title = "Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells",
abstract = "Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.",
author = "Jesang Ko and Kim, {In Sik} and Jang, {Sung Wuk} and Lee, {Young Han} and Shin, {Soon Young} and Min, {Do Sik} and Na, {Doe Sun}",
year = "2002",
month = "3",
day = "27",
doi = "10.1016/S0014-5793(02)02465-1",
language = "English",
volume = "515",
pages = "159--164",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

Ko, J, Kim, IS, Jang, SW, Lee, YH, Shin, SY, Min, DS & Na, DS 2002, 'Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells', FEBS Letters, vol. 515, no. 1-3, pp. 159-164. https://doi.org/10.1016/S0014-5793(02)02465-1

Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. / Ko, Jesang; Kim, In Sik; Jang, Sung Wuk; Lee, Young Han; Shin, Soon Young; Min, Do Sik; Na, Doe Sun.

In: FEBS Letters, Vol. 515, No. 1-3, 27.03.2002, p. 159-164.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells

AU - Ko, Jesang

AU - Kim, In Sik

AU - Jang, Sung Wuk

AU - Lee, Young Han

AU - Shin, Soon Young

AU - Min, Do Sik

AU - Na, Doe Sun

PY - 2002/3/27

Y1 - 2002/3/27

N2 - Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.

AB - Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.

UR - http://www.scopus.com/inward/record.url?scp=0037181475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037181475&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(02)02465-1

DO - 10.1016/S0014-5793(02)02465-1

M3 - Article

C2 - 11943214

AN - SCOPUS:0037181475

VL - 515

SP - 159

EP - 164

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -