Level of secreted HMGB1 correlates with severity of inflammation in chronic rhinosinusitis

Hyun Jin Min, Su Jin Kim, Tae Hoon Kim, Hyo Jin Chung, Joo Heon Yoon, Chang-Hoon Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective High mobility group box 1 (HMGB1) protein is a chromatin protein that functions as a proinflammatory cytokine when secreted in response to inflammatory stimuli. The purpose of this study was to determine the relationship between the HMGB1 level in nasal secretions and the severity of inflammation in chronic rhinosinusitis. Study Design This was a cross-sectional study. Methods Nasal secretions were obtained by irrigation of the affected sinonasal cavities with normal saline. Total 63 nasal lavage fluid samples were collected from 38 patients with chronic rhinosinusitis who underwent endoscopic sinus surgery. Levels of HMGB1 and tumor necrosis factor alpha, interleukin (IL)-1β, and IL-8 were determined by enzyme-linked immunoassay. Severity of inflammation was assessed by the Lund-Mackay scoring system, which is based on preoperative computed tomography scans. Concurrent medical disorders, presence of nasal polyps, septal deviation, and allergic rhinitis were also investigated. Results The level of HMGB1 in nasal lavage fluid was positively correlated with the Lund-Mackay score. The score was the only factor associated with HMGB1 by univariate and multivariate analysis. Other cytokines, with the exception of IL-8, were not correlated with the Lund-Mackay score. Conclusion Our results showed that HMGB1 is secreted into the extracellular area space in the upper airway, and HMGB1 levels in nasal lavage fluid correlate with severity of inflammation, as assessed by the Lund-Mackay staging system for chronic rhinosinusitis. These results provide evidence for HMGB1 as an inflammatory mediator associated with the severity of chronic rhinosinusitis. Level of Evidence N/A. Laryngoscope, 125:E225-E230, 2015

Original languageEnglish
Pages (from-to)E225-E230
JournalLaryngoscope
Volume125
Issue number7
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Nasal Lavage Fluid
Inflammation
Interleukin-8
Nose
Cytokines
HMGB1 Protein
Laryngoscopes
Nasal Polyps
Extracellular Space
Immunoenzyme Techniques
Interleukin-1
Chromatin
Multivariate Analysis
Tumor Necrosis Factor-alpha
Cross-Sectional Studies
Tomography
Proteins

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Min, Hyun Jin ; Kim, Su Jin ; Kim, Tae Hoon ; Chung, Hyo Jin ; Yoon, Joo Heon ; Kim, Chang-Hoon. / Level of secreted HMGB1 correlates with severity of inflammation in chronic rhinosinusitis. In: Laryngoscope. 2015 ; Vol. 125, No. 7. pp. E225-E230.
@article{e1937f581de34bfe9821563daacf7c3e,
title = "Level of secreted HMGB1 correlates with severity of inflammation in chronic rhinosinusitis",
abstract = "Objective High mobility group box 1 (HMGB1) protein is a chromatin protein that functions as a proinflammatory cytokine when secreted in response to inflammatory stimuli. The purpose of this study was to determine the relationship between the HMGB1 level in nasal secretions and the severity of inflammation in chronic rhinosinusitis. Study Design This was a cross-sectional study. Methods Nasal secretions were obtained by irrigation of the affected sinonasal cavities with normal saline. Total 63 nasal lavage fluid samples were collected from 38 patients with chronic rhinosinusitis who underwent endoscopic sinus surgery. Levels of HMGB1 and tumor necrosis factor alpha, interleukin (IL)-1β, and IL-8 were determined by enzyme-linked immunoassay. Severity of inflammation was assessed by the Lund-Mackay scoring system, which is based on preoperative computed tomography scans. Concurrent medical disorders, presence of nasal polyps, septal deviation, and allergic rhinitis were also investigated. Results The level of HMGB1 in nasal lavage fluid was positively correlated with the Lund-Mackay score. The score was the only factor associated with HMGB1 by univariate and multivariate analysis. Other cytokines, with the exception of IL-8, were not correlated with the Lund-Mackay score. Conclusion Our results showed that HMGB1 is secreted into the extracellular area space in the upper airway, and HMGB1 levels in nasal lavage fluid correlate with severity of inflammation, as assessed by the Lund-Mackay staging system for chronic rhinosinusitis. These results provide evidence for HMGB1 as an inflammatory mediator associated with the severity of chronic rhinosinusitis. Level of Evidence N/A. Laryngoscope, 125:E225-E230, 2015",
author = "Min, {Hyun Jin} and Kim, {Su Jin} and Kim, {Tae Hoon} and Chung, {Hyo Jin} and Yoon, {Joo Heon} and Chang-Hoon Kim",
year = "2015",
month = "7",
day = "1",
doi = "10.1002/lary.25172",
language = "English",
volume = "125",
pages = "E225--E230",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "7",

}

Level of secreted HMGB1 correlates with severity of inflammation in chronic rhinosinusitis. / Min, Hyun Jin; Kim, Su Jin; Kim, Tae Hoon; Chung, Hyo Jin; Yoon, Joo Heon; Kim, Chang-Hoon.

In: Laryngoscope, Vol. 125, No. 7, 01.07.2015, p. E225-E230.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Level of secreted HMGB1 correlates with severity of inflammation in chronic rhinosinusitis

AU - Min, Hyun Jin

AU - Kim, Su Jin

AU - Kim, Tae Hoon

AU - Chung, Hyo Jin

AU - Yoon, Joo Heon

AU - Kim, Chang-Hoon

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Objective High mobility group box 1 (HMGB1) protein is a chromatin protein that functions as a proinflammatory cytokine when secreted in response to inflammatory stimuli. The purpose of this study was to determine the relationship between the HMGB1 level in nasal secretions and the severity of inflammation in chronic rhinosinusitis. Study Design This was a cross-sectional study. Methods Nasal secretions were obtained by irrigation of the affected sinonasal cavities with normal saline. Total 63 nasal lavage fluid samples were collected from 38 patients with chronic rhinosinusitis who underwent endoscopic sinus surgery. Levels of HMGB1 and tumor necrosis factor alpha, interleukin (IL)-1β, and IL-8 were determined by enzyme-linked immunoassay. Severity of inflammation was assessed by the Lund-Mackay scoring system, which is based on preoperative computed tomography scans. Concurrent medical disorders, presence of nasal polyps, septal deviation, and allergic rhinitis were also investigated. Results The level of HMGB1 in nasal lavage fluid was positively correlated with the Lund-Mackay score. The score was the only factor associated with HMGB1 by univariate and multivariate analysis. Other cytokines, with the exception of IL-8, were not correlated with the Lund-Mackay score. Conclusion Our results showed that HMGB1 is secreted into the extracellular area space in the upper airway, and HMGB1 levels in nasal lavage fluid correlate with severity of inflammation, as assessed by the Lund-Mackay staging system for chronic rhinosinusitis. These results provide evidence for HMGB1 as an inflammatory mediator associated with the severity of chronic rhinosinusitis. Level of Evidence N/A. Laryngoscope, 125:E225-E230, 2015

AB - Objective High mobility group box 1 (HMGB1) protein is a chromatin protein that functions as a proinflammatory cytokine when secreted in response to inflammatory stimuli. The purpose of this study was to determine the relationship between the HMGB1 level in nasal secretions and the severity of inflammation in chronic rhinosinusitis. Study Design This was a cross-sectional study. Methods Nasal secretions were obtained by irrigation of the affected sinonasal cavities with normal saline. Total 63 nasal lavage fluid samples were collected from 38 patients with chronic rhinosinusitis who underwent endoscopic sinus surgery. Levels of HMGB1 and tumor necrosis factor alpha, interleukin (IL)-1β, and IL-8 were determined by enzyme-linked immunoassay. Severity of inflammation was assessed by the Lund-Mackay scoring system, which is based on preoperative computed tomography scans. Concurrent medical disorders, presence of nasal polyps, septal deviation, and allergic rhinitis were also investigated. Results The level of HMGB1 in nasal lavage fluid was positively correlated with the Lund-Mackay score. The score was the only factor associated with HMGB1 by univariate and multivariate analysis. Other cytokines, with the exception of IL-8, were not correlated with the Lund-Mackay score. Conclusion Our results showed that HMGB1 is secreted into the extracellular area space in the upper airway, and HMGB1 levels in nasal lavage fluid correlate with severity of inflammation, as assessed by the Lund-Mackay staging system for chronic rhinosinusitis. These results provide evidence for HMGB1 as an inflammatory mediator associated with the severity of chronic rhinosinusitis. Level of Evidence N/A. Laryngoscope, 125:E225-E230, 2015

UR - http://www.scopus.com/inward/record.url?scp=84932137234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84932137234&partnerID=8YFLogxK

U2 - 10.1002/lary.25172

DO - 10.1002/lary.25172

M3 - Article

VL - 125

SP - E225-E230

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 7

ER -