Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters

T. H. Tu, C. S. Kim, J. H. Kang, I. S. Nam-Goong, C. W. Nam, E. S. Kim, Y. I. Kim, J. I. Choi, T. Kawada, T. Goto, T. Park, J. H. Yoon Park, M. S. Choi, R. Yu

Research output: Contribution to journalArticle

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Abstract

Background:4-1BB, a member of the TNF receptor superfamily, has a role in various inflammatory pathologies through its interaction with 4-1BB ligand. We previously demonstrated that it participates in initiating and promoting obesity-induced adipose inflammation in a rodent model.Objective:In this study, we examined whether 4-1BB is related to obesity-induced adipose inflammation and metabolic parameters in humans.Methods:A total of 50 subjects, 25 obese (body mass index (BMI)≥25 kg m -2) and 25 lean (BMI<23 kg m -2) participated in the study. The levels of 4-1BB transcripts and soluble 4-1BB protein (s4-1BB) in subcutaneous adipose tissue were measured by quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Inflammatory and metabolic parameters were measured by enzymatic analysis and immunoassay.Results:Obese subjects had higher levels of both 4-1BB transcripts and s4-1BB protein in subcutaneous adipose tissue than lean controls, and the levels were correlated with BMI and the expression of inflammatory markers, as well as with serum metabolic parameters. Moreover, s4-1BB was released from human adipocytes, and elicited chemotactic responses from human monocytes/T cells as well as enhancing their inflammatory activity, indicating that it may promote human adipose inflammation.Discussion:Our data demonstrate that elevated levels of 4-1BB transcripts and s4-1BB in adipose tissue are closely associated with obesity-induced inflammation and metabolic dysregulation. They suggest that both 4-1BB transcripts and s4-1BB could serve as novel biomarkers and/or therapeutic targets for obesity-induced inflammation and metabolic syndrome in humans.

Original languageEnglish
Pages (from-to)1075-1082
Number of pages8
JournalInternational Journal of Obesity
Volume38
Issue number8
DOIs
Publication statusPublished - 2014 Aug

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Adipose Tissue
Inflammation
Obesity
Proteins
Body Mass Index
Subcutaneous Fat
4-1BB Ligand
Tumor Necrosis Factor Receptors
Immunoassay
Adipocytes
Real-Time Polymerase Chain Reaction
Monocytes
Rodentia
Biomarkers
Enzyme-Linked Immunosorbent Assay
Pathology
T-Lymphocytes
Serum
Therapeutics

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Tu, T. H. ; Kim, C. S. ; Kang, J. H. ; Nam-Goong, I. S. ; Nam, C. W. ; Kim, E. S. ; Kim, Y. I. ; Choi, J. I. ; Kawada, T. ; Goto, T. ; Park, T. ; Yoon Park, J. H. ; Choi, M. S. ; Yu, R. / Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters. In: International Journal of Obesity. 2014 ; Vol. 38, No. 8. pp. 1075-1082.
@article{d860b7abc17d40f9948e4ada7cb9ebdf,
title = "Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters",
abstract = "Background:4-1BB, a member of the TNF receptor superfamily, has a role in various inflammatory pathologies through its interaction with 4-1BB ligand. We previously demonstrated that it participates in initiating and promoting obesity-induced adipose inflammation in a rodent model.Objective:In this study, we examined whether 4-1BB is related to obesity-induced adipose inflammation and metabolic parameters in humans.Methods:A total of 50 subjects, 25 obese (body mass index (BMI)≥25 kg m -2) and 25 lean (BMI<23 kg m -2) participated in the study. The levels of 4-1BB transcripts and soluble 4-1BB protein (s4-1BB) in subcutaneous adipose tissue were measured by quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Inflammatory and metabolic parameters were measured by enzymatic analysis and immunoassay.Results:Obese subjects had higher levels of both 4-1BB transcripts and s4-1BB protein in subcutaneous adipose tissue than lean controls, and the levels were correlated with BMI and the expression of inflammatory markers, as well as with serum metabolic parameters. Moreover, s4-1BB was released from human adipocytes, and elicited chemotactic responses from human monocytes/T cells as well as enhancing their inflammatory activity, indicating that it may promote human adipose inflammation.Discussion:Our data demonstrate that elevated levels of 4-1BB transcripts and s4-1BB in adipose tissue are closely associated with obesity-induced inflammation and metabolic dysregulation. They suggest that both 4-1BB transcripts and s4-1BB could serve as novel biomarkers and/or therapeutic targets for obesity-induced inflammation and metabolic syndrome in humans.",
author = "Tu, {T. H.} and Kim, {C. S.} and Kang, {J. H.} and Nam-Goong, {I. S.} and Nam, {C. W.} and Kim, {E. S.} and Kim, {Y. I.} and Choi, {J. I.} and T. Kawada and T. Goto and T. Park and {Yoon Park}, {J. H.} and Choi, {M. S.} and R. Yu",
year = "2014",
month = "8",
doi = "10.1038/ijo.2013.222",
language = "English",
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Tu, TH, Kim, CS, Kang, JH, Nam-Goong, IS, Nam, CW, Kim, ES, Kim, YI, Choi, JI, Kawada, T, Goto, T, Park, T, Yoon Park, JH, Choi, MS & Yu, R 2014, 'Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters', International Journal of Obesity, vol. 38, no. 8, pp. 1075-1082. https://doi.org/10.1038/ijo.2013.222

Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters. / Tu, T. H.; Kim, C. S.; Kang, J. H.; Nam-Goong, I. S.; Nam, C. W.; Kim, E. S.; Kim, Y. I.; Choi, J. I.; Kawada, T.; Goto, T.; Park, T.; Yoon Park, J. H.; Choi, M. S.; Yu, R.

In: International Journal of Obesity, Vol. 38, No. 8, 08.2014, p. 1075-1082.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Levels of 4-1BB transcripts and soluble 4-1BB protein are elevated in the adipose tissue of human obese subjects and are associated with inflammatory and metabolic parameters

AU - Tu, T. H.

AU - Kim, C. S.

AU - Kang, J. H.

AU - Nam-Goong, I. S.

AU - Nam, C. W.

AU - Kim, E. S.

AU - Kim, Y. I.

AU - Choi, J. I.

AU - Kawada, T.

AU - Goto, T.

AU - Park, T.

AU - Yoon Park, J. H.

AU - Choi, M. S.

AU - Yu, R.

PY - 2014/8

Y1 - 2014/8

N2 - Background:4-1BB, a member of the TNF receptor superfamily, has a role in various inflammatory pathologies through its interaction with 4-1BB ligand. We previously demonstrated that it participates in initiating and promoting obesity-induced adipose inflammation in a rodent model.Objective:In this study, we examined whether 4-1BB is related to obesity-induced adipose inflammation and metabolic parameters in humans.Methods:A total of 50 subjects, 25 obese (body mass index (BMI)≥25 kg m -2) and 25 lean (BMI<23 kg m -2) participated in the study. The levels of 4-1BB transcripts and soluble 4-1BB protein (s4-1BB) in subcutaneous adipose tissue were measured by quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Inflammatory and metabolic parameters were measured by enzymatic analysis and immunoassay.Results:Obese subjects had higher levels of both 4-1BB transcripts and s4-1BB protein in subcutaneous adipose tissue than lean controls, and the levels were correlated with BMI and the expression of inflammatory markers, as well as with serum metabolic parameters. Moreover, s4-1BB was released from human adipocytes, and elicited chemotactic responses from human monocytes/T cells as well as enhancing their inflammatory activity, indicating that it may promote human adipose inflammation.Discussion:Our data demonstrate that elevated levels of 4-1BB transcripts and s4-1BB in adipose tissue are closely associated with obesity-induced inflammation and metabolic dysregulation. They suggest that both 4-1BB transcripts and s4-1BB could serve as novel biomarkers and/or therapeutic targets for obesity-induced inflammation and metabolic syndrome in humans.

AB - Background:4-1BB, a member of the TNF receptor superfamily, has a role in various inflammatory pathologies through its interaction with 4-1BB ligand. We previously demonstrated that it participates in initiating and promoting obesity-induced adipose inflammation in a rodent model.Objective:In this study, we examined whether 4-1BB is related to obesity-induced adipose inflammation and metabolic parameters in humans.Methods:A total of 50 subjects, 25 obese (body mass index (BMI)≥25 kg m -2) and 25 lean (BMI<23 kg m -2) participated in the study. The levels of 4-1BB transcripts and soluble 4-1BB protein (s4-1BB) in subcutaneous adipose tissue were measured by quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Inflammatory and metabolic parameters were measured by enzymatic analysis and immunoassay.Results:Obese subjects had higher levels of both 4-1BB transcripts and s4-1BB protein in subcutaneous adipose tissue than lean controls, and the levels were correlated with BMI and the expression of inflammatory markers, as well as with serum metabolic parameters. Moreover, s4-1BB was released from human adipocytes, and elicited chemotactic responses from human monocytes/T cells as well as enhancing their inflammatory activity, indicating that it may promote human adipose inflammation.Discussion:Our data demonstrate that elevated levels of 4-1BB transcripts and s4-1BB in adipose tissue are closely associated with obesity-induced inflammation and metabolic dysregulation. They suggest that both 4-1BB transcripts and s4-1BB could serve as novel biomarkers and/or therapeutic targets for obesity-induced inflammation and metabolic syndrome in humans.

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DO - 10.1038/ijo.2013.222

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JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

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