Light-induced cell separation in a tailored optical landscape

L. Paterson; E. Papagiakoumou; G. Milne; V. Garćs-Chávez; S. A. Tatarkova; W. Sibbett; F. J. Gunn-Moore; P. E. Bryant; A. C. Riches; K. Dholakia

doi:10.1063/1.2045548

Light-induced cell separation in a tailored optical landscape

L. Paterson, E. Papagiakoumou, G. Milne, V. Garćs-Chávez, S. A. Tatarkova, W. Sibbett, F. J. Gunn-Moore, P. E. Bryant, A. C. Riches, K. Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

We demonstrate passive optical sorting of cell populations in the absence of any externally driven fluid flow. Specifically, we report the movement of erythrocytes and lymphocytes in an optical landscape, consisting of a circularly symmetric light pattern created by a Bessel light beam. These distinct cell populations move, spontaneously and differentially, across the underlying periodic optical landscape. Thus, we were able to separate lymphocytes from a mixed population of cells containing erythrocytes and then collect the lymphocytes in a microcapillary reservoir. We also demonstrate an enhanced form of this separation that exploits the polarizability of silica microspheres by attaching spheres coated with antibodies to cell surface markers to a subpopulation of lymphocytes. These techniques may be applied using standard laboratory apparatus.

Original language	English
Article number	123901
Pages (from-to)	1-3
Number of pages	3
Journal	Applied Physics Letters
Volume	87
Issue number	12
DOIs	https://doi.org/10.1063/1.2045548
Publication status	Published - 2005 Sep 19

Bibliographical note

Funding Information:
The authors thank G. Spalding, D. McGloin, E. Brown, C. Briscoe, M.L. McRobbie, and M. Comrie for useful discussions. This work is funded by the Scottish Higher Education Funding Council, the Medical Research Council, and the European Science Foundation EUROCORES Programme SONS , project NOMSAN, supported by funds from the UK Engineering and Physical Sciences Programme and the EC Sixth Framework Programme. One of the authors (E.P.) acknowledges support from the “Heraclitus” project, cofunded by the European Social Fund (75%) and National Resources (25%).

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

Access to Document

10.1063/1.2045548

Cite this

@article{2d7cea85015447e08847e21cf80be646,

title = "Light-induced cell separation in a tailored optical landscape",

abstract = "We demonstrate passive optical sorting of cell populations in the absence of any externally driven fluid flow. Specifically, we report the movement of erythrocytes and lymphocytes in an optical landscape, consisting of a circularly symmetric light pattern created by a Bessel light beam. These distinct cell populations move, spontaneously and differentially, across the underlying periodic optical landscape. Thus, we were able to separate lymphocytes from a mixed population of cells containing erythrocytes and then collect the lymphocytes in a microcapillary reservoir. We also demonstrate an enhanced form of this separation that exploits the polarizability of silica microspheres by attaching spheres coated with antibodies to cell surface markers to a subpopulation of lymphocytes. These techniques may be applied using standard laboratory apparatus.",

author = "L. Paterson and E. Papagiakoumou and G. Milne and V. Gar{\'c}s-Ch{\'a}vez and Tatarkova, {S. A.} and W. Sibbett and Gunn-Moore, {F. J.} and Bryant, {P. E.} and Riches, {A. C.} and K. Dholakia",

note = "Funding Information: The authors thank G. Spalding, D. McGloin, E. Brown, C. Briscoe, M.L. McRobbie, and M. Comrie for useful discussions. This work is funded by the Scottish Higher Education Funding Council, the Medical Research Council, and the European Science Foundation EUROCORES Programme SONS , project NOMSAN, supported by funds from the UK Engineering and Physical Sciences Programme and the EC Sixth Framework Programme. One of the authors (E.P.) acknowledges support from the “Heraclitus” project, cofunded by the European Social Fund (75%) and National Resources (25%). ",

year = "2005",

month = sep,

day = "19",

doi = "10.1063/1.2045548",

language = "English",

volume = "87",

pages = "1--3",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

number = "12",

}

TY - JOUR

T1 - Light-induced cell separation in a tailored optical landscape

AU - Paterson, L.

AU - Papagiakoumou, E.

AU - Milne, G.

AU - Garćs-Chávez, V.

AU - Tatarkova, S. A.

AU - Sibbett, W.

AU - Gunn-Moore, F. J.

AU - Bryant, P. E.

AU - Riches, A. C.

AU - Dholakia, K.

N1 - Funding Information: The authors thank G. Spalding, D. McGloin, E. Brown, C. Briscoe, M.L. McRobbie, and M. Comrie for useful discussions. This work is funded by the Scottish Higher Education Funding Council, the Medical Research Council, and the European Science Foundation EUROCORES Programme SONS , project NOMSAN, supported by funds from the UK Engineering and Physical Sciences Programme and the EC Sixth Framework Programme. One of the authors (E.P.) acknowledges support from the “Heraclitus” project, cofunded by the European Social Fund (75%) and National Resources (25%).

PY - 2005/9/19

Y1 - 2005/9/19

N2 - We demonstrate passive optical sorting of cell populations in the absence of any externally driven fluid flow. Specifically, we report the movement of erythrocytes and lymphocytes in an optical landscape, consisting of a circularly symmetric light pattern created by a Bessel light beam. These distinct cell populations move, spontaneously and differentially, across the underlying periodic optical landscape. Thus, we were able to separate lymphocytes from a mixed population of cells containing erythrocytes and then collect the lymphocytes in a microcapillary reservoir. We also demonstrate an enhanced form of this separation that exploits the polarizability of silica microspheres by attaching spheres coated with antibodies to cell surface markers to a subpopulation of lymphocytes. These techniques may be applied using standard laboratory apparatus.

AB - We demonstrate passive optical sorting of cell populations in the absence of any externally driven fluid flow. Specifically, we report the movement of erythrocytes and lymphocytes in an optical landscape, consisting of a circularly symmetric light pattern created by a Bessel light beam. These distinct cell populations move, spontaneously and differentially, across the underlying periodic optical landscape. Thus, we were able to separate lymphocytes from a mixed population of cells containing erythrocytes and then collect the lymphocytes in a microcapillary reservoir. We also demonstrate an enhanced form of this separation that exploits the polarizability of silica microspheres by attaching spheres coated with antibodies to cell surface markers to a subpopulation of lymphocytes. These techniques may be applied using standard laboratory apparatus.

UR - http://www.scopus.com/inward/record.url?scp=24644480406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24644480406&partnerID=8YFLogxK

U2 - 10.1063/1.2045548

DO - 10.1063/1.2045548

M3 - Article

AN - SCOPUS:24644480406

VL - 87

SP - 1

EP - 3

JO - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 12

M1 - 123901

ER -

Light-induced cell separation in a tailored optical landscape

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this