Light-Propelled Nanorobots for Facial Titanium Implants Biofilms Removal

Martina Ussia, Mario Urso, Stepan Kment, Tatiana Fialova, Karel Klima, Kristyna Dolezelikova, Martin Pumera

Research output: Contribution to journalArticlepeer-review

Abstract

Titanium miniplates are biocompatible materials used in modern oral and maxillofacial surgery to treat facial bone fractures. However, plate removal is often required due to implant complications. Among them, a biofilm formation on an infected miniplate is associated with severe inflammation, which frequently results in implant failure. In light of this, new strategies to control or treat oral bacterial biofilm are of high interest. Herein, the authors exploit the ability of nanorobots against multispecies bacterial biofilm grown onto facial commercial titanium miniplate implants to simulate pathogenic conditions of the oral microenvironment. The strategy is based on the use of light-driven self-propelled tubular black-TiO2/Ag nanorobots, that unlike traditional ones, exhibit an extended absorption and motion actuation from UV to the visible-light range. The motion analysis is performed separately over UV, blue, and green light irradiation and shows different motion behaviors, including a fast rotational motion that decreases with increasing wavelengths. The biomass reduction is monitored by evaluating LIVE/DEAD fluorescent and digital microscope images of bacterial biofilm treated with the nanorobots under motion/no-motion conditions. The current study and the obtained results can bring significant improvements for effective therapy of infected metallic miniplates by biofilm.

Original languageEnglish
Article number2200708
JournalSmall
Volume18
Issue number22
DOIs
Publication statusPublished - 2022 Jun 2

Bibliographical note

Funding Information:
This work was supported by the ESF under the project CZ.02.2.69/0.0/0.0/18_053/0016962. CzechNanoLab project LM2018110 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements/sample fabrication at CEITEC Nano Research Infrastructure. M.P. was supported by Ministry of Education, Youth and Sports (Czech Republic) grant LL2002 under ERC CZ program and Ministry of Health of the Czech Republic (grant NU21-08-00407). M.Ur. acknowledges the financial support by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 101038066. S.K. acknowledges the funding from the Czech Science Foundation, project GA CR – EXPRO, 19–27454X. K.D. thanks ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No. CZ.02.1.01/0.0/0.0/16_025/0007314).

Funding Information:
This work was supported by the ESF under the project CZ.02.2.69/0.0/0.0/18_053/0016962. CzechNanoLab project LM2018110 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements/sample fabrication at CEITEC Nano Research Infrastructure. M.P. was supported by Ministry of Education, Youth and Sports (Czech Republic) grant LL2002 under ERC CZ program and Ministry of Health of the Czech Republic (grant NU21‐08‐00407). M.Ur. acknowledges the financial support by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska‐Curie grant agreement No. 101038066. S.K. acknowledges the funding from the Czech Science Foundation, project GA CR – EXPRO, 19–27454X. K.D. thanks ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No. CZ.02.1.01/0.0/0.0/16_025/0007314).

Publisher Copyright:
© 2022 Wiley-VCH GmbH.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Chemistry(all)
  • Biomaterials
  • Materials Science(all)

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