Light-sheet fluorescence microscopy with structured light

Jonathan Nylk, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Light-sheet microscopy has emerged as a powerful approach for long-term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximized while retaining high resolution. There are additional advantages of minimizing photodamage.

Original languageEnglish
Title of host publicationNeurophotonics and Biomedical Spectroscopy
PublisherElsevier
Pages477-501
Number of pages25
ISBN (Electronic)9780323480673
ISBN (Print)9780323480680
DOIs
Publication statusPublished - 2018 Jan 1

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Materials Science(all)

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  • Cite this

    Nylk, J., & Dholakia, K. (2018). Light-sheet fluorescence microscopy with structured light. In Neurophotonics and Biomedical Spectroscopy (pp. 477-501). Elsevier. https://doi.org/10.1016/B978-0-323-48067-3.00018-4