Light-sheet microscopy using an Airy beam

Tom Vettenburg, Heather I.C. Dalgarno, Jonathan Nylk, Clara Coll-Lladó, David E.K. Ferrier, Tomáš Čižmár, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

Original languageEnglish
Pages (from-to)541-544
Number of pages4
JournalNature Methods
Volume11
Issue number5
DOIs
Publication statusPublished - 2014 May

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Microscopy
Microscopic examination
Light
Fluorescence
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Vettenburg, T., Dalgarno, H. I. C., Nylk, J., Coll-Lladó, C., Ferrier, D. E. K., Čižmár, T., ... Dholakia, K. (2014). Light-sheet microscopy using an Airy beam. Nature Methods, 11(5), 541-544. https://doi.org/10.1038/nmeth.2922
Vettenburg, Tom ; Dalgarno, Heather I.C. ; Nylk, Jonathan ; Coll-Lladó, Clara ; Ferrier, David E.K. ; Čižmár, Tomáš ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Light-sheet microscopy using an Airy beam. In: Nature Methods. 2014 ; Vol. 11, No. 5. pp. 541-544.
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Vettenburg, T, Dalgarno, HIC, Nylk, J, Coll-Lladó, C, Ferrier, DEK, Čižmár, T, Gunn-Moore, FJ & Dholakia, K 2014, 'Light-sheet microscopy using an Airy beam', Nature Methods, vol. 11, no. 5, pp. 541-544. https://doi.org/10.1038/nmeth.2922

Light-sheet microscopy using an Airy beam. / Vettenburg, Tom; Dalgarno, Heather I.C.; Nylk, Jonathan; Coll-Lladó, Clara; Ferrier, David E.K.; Čižmár, Tomáš; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: Nature Methods, Vol. 11, No. 5, 05.2014, p. 541-544.

Research output: Contribution to journalArticle

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Vettenburg T, Dalgarno HIC, Nylk J, Coll-Lladó C, Ferrier DEK, Čižmár T et al. Light-sheet microscopy using an Airy beam. Nature Methods. 2014 May;11(5):541-544. https://doi.org/10.1038/nmeth.2922