Light sheet microscopy with acoustic sample confinement

Zhengyi Yang, Katy L.H. Cole, Yongqiang Qiu, Ildikó M.L. Somorjai, Philip Wijesinghe, Jonathan Nylk, Sandy Cochran, Gabriel C. Spalding, David A. Lyons, Kishan Dholakia

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Contactless sample confinement would enable a whole host of new studies in developmental biology and neuroscience, in particular, when combined with long-term, wide-field optical imaging. To achieve this goal, we demonstrate a contactless acoustic gradient force trap for sample confinement in light sheet microscopy. Our approach allows the integration of real-time environmentally controlled experiments with wide-field low photo-toxic imaging, which we demonstrate on a variety of marine animal embryos and larvae. To illustrate the key advantages of our approach, we provide quantitative data for the dynamic response of the heartbeat of zebrafish larvae to verapamil and norepinephrine, which are known to affect cardiovascular function. Optical flow analysis allows us to explore the cardiac cycle of the zebrafish and determine the changes in contractile volume within the heart. Overcoming the restrictions of sample immobilisation and mounting can open up a broad range of studies, with real-time drug-based assays and biomechanical analyses.

Original languageEnglish
Article number669
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Acoustic Microscopy
Zebrafish
larvae
Larva
Microscopic examination
Acoustics
microscopy
Imaging techniques
Light
Developmental Biology
Cardiac Volume
acoustics
Optical flows
Poisons
Optical Imaging
Neurosciences
norepinephrine
Verapamil
Mountings
Immobilization

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Yang, Z., Cole, K. L. H., Qiu, Y., Somorjai, I. M. L., Wijesinghe, P., Nylk, J., ... Dholakia, K. (2019). Light sheet microscopy with acoustic sample confinement. Nature communications, 10(1), [669]. https://doi.org/10.1038/s41467-019-08514-5
Yang, Zhengyi ; Cole, Katy L.H. ; Qiu, Yongqiang ; Somorjai, Ildikó M.L. ; Wijesinghe, Philip ; Nylk, Jonathan ; Cochran, Sandy ; Spalding, Gabriel C. ; Lyons, David A. ; Dholakia, Kishan. / Light sheet microscopy with acoustic sample confinement. In: Nature communications. 2019 ; Vol. 10, No. 1.
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Yang, Z, Cole, KLH, Qiu, Y, Somorjai, IML, Wijesinghe, P, Nylk, J, Cochran, S, Spalding, GC, Lyons, DA & Dholakia, K 2019, 'Light sheet microscopy with acoustic sample confinement', Nature communications, vol. 10, no. 1, 669. https://doi.org/10.1038/s41467-019-08514-5

Light sheet microscopy with acoustic sample confinement. / Yang, Zhengyi; Cole, Katy L.H.; Qiu, Yongqiang; Somorjai, Ildikó M.L.; Wijesinghe, Philip; Nylk, Jonathan; Cochran, Sandy; Spalding, Gabriel C.; Lyons, David A.; Dholakia, Kishan.

In: Nature communications, Vol. 10, No. 1, 669, 01.12.2019.

Research output: Contribution to journalArticle

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Yang Z, Cole KLH, Qiu Y, Somorjai IML, Wijesinghe P, Nylk J et al. Light sheet microscopy with acoustic sample confinement. Nature communications. 2019 Dec 1;10(1). 669. https://doi.org/10.1038/s41467-019-08514-5