Light-sheet microscopy with attenuation-compensated propagation-invariant beams

Jonathan Nylk, Kaley McCluskey, Miguel A. Preciado, Michael Mazilu, Zhengyi Yang, Frank J. Gunn-Moore, Sanya Aggarwal, Javier A. Tello, David E.K. Ferrier, Kishan Dholakia

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Scattering and absorption limit the penetration of optical fields into tissue. We demonstrate a new approach for increased depth penetration in light-sheet microscopy: attenuation-compensation of the light field. This tailors an exponential intensity increase along the illuminating propagation-invariant field, enabling the redistribution of intensity strategically within a sample to maximize signal and minimize irradiation. A key attribute of this method is that only minimal knowledge of the specimen transmission properties is required. We numerically quantify the imaging capabilities of attenuation-compensated Airy and Bessel light sheets, showing that increased depth penetration is gained without compromising any other beam attributes. This powerful yet straightforward concept, combined with the self-healing properties of the propagation-invariant field, improves the contrast-to-noise ratio of light-sheet microscopy up to eightfold across the entire field of view in thick biological specimens. This improvement can significantly increase the imaging capabilities of light-sheet microscopy techniques using Airy, Bessel, and other propagation-invariant beam types, paving the way for widespread uptake by the biomedical community.

Original languageEnglish
Article numbereaar4817
JournalScience Advances
Volume4
Issue number4
DOIs
Publication statusPublished - 2018 Apr 6

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Microscopy
Light
Noise

All Science Journal Classification (ASJC) codes

  • General

Cite this

Nylk, J., McCluskey, K., Preciado, M. A., Mazilu, M., Yang, Z., Gunn-Moore, F. J., ... Dholakia, K. (2018). Light-sheet microscopy with attenuation-compensated propagation-invariant beams. Science Advances, 4(4), [eaar4817]. https://doi.org/10.1126/sciadv.aar4817
Nylk, Jonathan ; McCluskey, Kaley ; Preciado, Miguel A. ; Mazilu, Michael ; Yang, Zhengyi ; Gunn-Moore, Frank J. ; Aggarwal, Sanya ; Tello, Javier A. ; Ferrier, David E.K. ; Dholakia, Kishan. / Light-sheet microscopy with attenuation-compensated propagation-invariant beams. In: Science Advances. 2018 ; Vol. 4, No. 4.
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Nylk, J, McCluskey, K, Preciado, MA, Mazilu, M, Yang, Z, Gunn-Moore, FJ, Aggarwal, S, Tello, JA, Ferrier, DEK & Dholakia, K 2018, 'Light-sheet microscopy with attenuation-compensated propagation-invariant beams', Science Advances, vol. 4, no. 4, eaar4817. https://doi.org/10.1126/sciadv.aar4817

Light-sheet microscopy with attenuation-compensated propagation-invariant beams. / Nylk, Jonathan; McCluskey, Kaley; Preciado, Miguel A.; Mazilu, Michael; Yang, Zhengyi; Gunn-Moore, Frank J.; Aggarwal, Sanya; Tello, Javier A.; Ferrier, David E.K.; Dholakia, Kishan.

In: Science Advances, Vol. 4, No. 4, eaar4817, 06.04.2018.

Research output: Contribution to journalArticle

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Nylk J, McCluskey K, Preciado MA, Mazilu M, Yang Z, Gunn-Moore FJ et al. Light-sheet microscopy with attenuation-compensated propagation-invariant beams. Science Advances. 2018 Apr 6;4(4). eaar4817. https://doi.org/10.1126/sciadv.aar4817