Light-stabilized FHA2 suppresses miRNA biogenesis through interactions with DCL1 and HYL1

Seung Jun Park, Suk Won Choi, Gu Min Kim, Christian Møller, Hyun Sook Pai, Seong Wook Yang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The precise regulation of microRNA (miRNA) biogenesis is crucial for plant development, which requires core microprocessors and many fine tuners to coordinate their miRNA processing activity/specificity in fluctuating cellular environments. During de-etiolation, light triggers a dramatic accumulation of core microprocessors and primary miRNAs (pri-miRNAs) but decreases pri-miRNA processing activity, resulting in relatively constant miRNA levels. The mechanisms underlying these seemingly contradictory regulatory changes remain unclear. In this study, we identified forkhead-associated domain 2 (FHA2) as a light-stabilized suppressor of miRNA biogenesis. We found that FHA2 deficiency increased the level of mature miRNAs, accompanied by a reduction in pri-miRNAs and target mRNAs. Biochemical assays showed that FHA2 associates with the core microprocessors DCL1, HYL1, and SE, forming a complex to suppress their pri-miRNA processing activity. Further analyses revealed that FHA2 promotes HYL1 binding but inhibits the binding of DCL1-PAZ-RNase-RNA-binding domains (DCL1-PRR) to miRNAs, whereas FHA2 does not directly bind to these RNAs. Interestingly, we found that FHA2 protein is unstable in the dark but stabilized by light during de-etiolation. Consistently, disruption of FHA led to defects in light-triggered changes in miRNA expression and reduced the survival rate of de-etiolated seedlings after prolonged light deprivation. Collectively, these data suggest that FHA2 is a novel light-stabilized suppressor of miRNA biogenesis and plays a role in fine-tuning miRNA processing during de-etiolation.

Original languageEnglish
JournalMolecular Plant
DOIs
Publication statusAccepted/In press - 2021

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, and Future Planning (NRF-2020R1A2B5B01002592 and 2018R1A6A1A03025607) to S.W.Y. This research was supported by the following grants to HSP: Cooperative Research Program for Agriculture Science & Technology Development (Project number PJ013227 of Systems & Synthetic Agrobiotech Center) from the Rural Development Administration and Mid-Career Researcher Program (NRF-2019R1A2B5B01069573) from the National Research Foundation (NRF) of the Republic of Korea. This research was supported in part by Brain Korea 21 (BK21) PLUS program fellowship awards to S.J.P., S.W.C., and G.M.K. This study was supported by a faculty research grant of Yonsei University department of systems biology.

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT , and Future Planning ( NRF-2020R1A2B5B01002592 and 2018R1A6A1A03025607 ) to S.W.Y. This research was supported by the following grants to HSP: Cooperative Research Program for Agriculture Science & Technology Development (Project number PJ013227 of Systems & Synthetic Agrobiotech Center) from the Rural Development Administration and Mid-Career Researcher Program ( NRF-2019R1A2B5B01069573 ) from the National Research Foundation (NRF) of the Republic of Korea. This research was supported in part by Brain Korea 21 (BK21) PLUS program fellowship awards to S.J.P., S.W.C., and G.M.K. This study was supported by a faculty research grant of Yonsei University department of systems biology.

Publisher Copyright:
© 2021 The Author

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

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