Lineage of non-cranial neural crest cell in the dental mesenchyme

Using a lacZ reporter gene during early tooth development

Sung Won Cho, Heui Jung Hwang, Jae Young Kim, Wu Chul Song, Soo Jin Song, Hitoshi Yamamoto, Hansung Jung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The tooth is one of the ectodermal organs controlled by reciprocal interactions between the epithelium and the mesenchyme. Mesenchymal cells in the developing tooth, so-called dental mesenchymal cells, are derived from two different origins: the cranial neural crest (CNC) and the non-CNC. These CNC-derived cells migrate, proliferate and differentiate into odontoblasts, cementoblasts, fibroblasts, osteoblasts and chondroblasts. Tooth germs of wild-type mice were transplanted into the kidney of adult lacZ-transgenic mice. After 1 week of transplantation, a few lacZ-expressing cells and many red blood cells were found near or inside the blood vessels in the pulp of wild-type tooth germs. This result shows that circulating cells of the adult host could invade the dental pulp during tooth development, through the blood vessels, and be a part of dental pulp tissue. Therefore, it can be suggested that these circulating progenitor cells could be the origin of non-CNC-derived cells in tooth germ and their migration pathways would be the blood vessels invading the dental pulp during tooth development. If variations of this experiment were suitably adjusted, such as the embryonic stage of the tooth germ, duration of transplantation, etc., this transplantation experiment using adult lacZ-transgenic mice could be a good system to reveal the origin and migration pathway of cells in developing organs as well as in dental mesenchymal cells.

Original languageEnglish
Pages (from-to)567-571
Number of pages5
JournalJournal of Electron Microscopy
Volume52
Issue number6
DOIs
Publication statusPublished - 2003 Dec 1

Fingerprint

Lac Operon
Neural Crest
Mesoderm
teeth
Reporter Genes
genes
Tooth Germ
Pulp
Tooth
Blood vessels
Genes
Dental Pulp
cells
Blood Vessels
transplantation
Transplantation
blood vessels
Transgenic Mice
mice
Osteoblasts

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

Cho, Sung Won ; Hwang, Heui Jung ; Kim, Jae Young ; Song, Wu Chul ; Song, Soo Jin ; Yamamoto, Hitoshi ; Jung, Hansung. / Lineage of non-cranial neural crest cell in the dental mesenchyme : Using a lacZ reporter gene during early tooth development. In: Journal of Electron Microscopy. 2003 ; Vol. 52, No. 6. pp. 567-571.
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Lineage of non-cranial neural crest cell in the dental mesenchyme : Using a lacZ reporter gene during early tooth development. / Cho, Sung Won; Hwang, Heui Jung; Kim, Jae Young; Song, Wu Chul; Song, Soo Jin; Yamamoto, Hitoshi; Jung, Hansung.

In: Journal of Electron Microscopy, Vol. 52, No. 6, 01.12.2003, p. 567-571.

Research output: Contribution to journalArticle

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