Lipid-like nanoparticles for small interfering RNA delivery to endothelial cells

Seung Woo Cho, Michael Goldberg, Sun Mi Son, Qiaobing Xu, Fan Yang, Ying Mei, Said Bogatyrev, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Here, nanoparticles composed of lipid-like materials (lipidoids) to facilitate non-viral delivery of small interfering RNA (siRNA) to endothelial cells (ECs) are developed. Nanoparticles composed of siRNA and lipidoids with small size (∼200 nm) and positive charge (∼34 mV) are formed by self-assembly of lipidoids and siRNA. Ten lipidoids are synthesized and screened for their ability to facilitate the delivery of siRNA into ECs. Particles composed of leading lipidoids show significantly better delivery to ECs than a leading commercially available transfection reagent, Lipofectamine 2000. As a model of potential therapeutic application, nanoparticles composed of the top performing lipidoid, NA114, are studied for their ability to deliver siRNA targeting anti-angiogenic factor (SHP-1) to human ECs. Silencing of SHP-1 expression significantly enhances EC proliferation and decreases EC apoptosis under a simulated ischemic condition.

Original languageEnglish
Pages (from-to)3112-3118
Number of pages7
JournalAdvanced Functional Materials
Volume19
Issue number19
DOIs
Publication statusPublished - 2009 Oct 9

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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  • Cite this

    Cho, S. W., Goldberg, M., Son, S. M., Xu, Q., Yang, F., Mei, Y., Bogatyrev, S., Langer, R., & Anderson, D. G. (2009). Lipid-like nanoparticles for small interfering RNA delivery to endothelial cells. Advanced Functional Materials, 19(19), 3112-3118. https://doi.org/10.1002/adfm.200900519