Lipopolysaccharide temporarily impairs sphincter of Oddi motility

Joseph J. Cullen, HyoJin Park, Kimberly S. Ephgrave, Jeffrey L. Conklin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of our study was to determine the effect of lipopolysaccharide (LPS) on sphincter of Oddi (SO) motility. Opossums received saline, Escherichia coli LPS (1.0 mg/kg), or E. coli LPS (1.0 mg/kg) and aminoguanidine (50 mg/kg), and the SO was removed 6-24 h later. At 12 h LPS decreased electrical field stimulation (EFS)-induced relaxation and increased baseline tone. These changes were reversed when the animals were pretreated with aminoguanidine. The dose-dependent decrease in EFS-induced relaxation by Nω-nitro-L-arginine was impaired after LPS, but not in animals that received LPS and aminoguanidine. The impaired EFS-induced relaxation after LPS was reversed when L-arginine was added to the tissue bath. Serum levels of NO2-/NO3- were increased with LPS as compared to saline or both LPS and aminoguanidine. Inducible nitric oxide synthase mRNA was readily seen in SO segments after LPS. LPS impairs EFS-induced relaxation and increases baseline tone of the SO. The effects of LPS on SO motility appear to be mediated by nitric oxide.

Original languageEnglish
Pages (from-to)547-554
Number of pages8
JournalNitric Oxide - Biology and Chemistry
Volume5
Issue number6
DOIs
Publication statusPublished - 2001 Jan 1

Fingerprint

Sphincter of Oddi
Lipopolysaccharides
Electric Stimulation
Escherichia coli
Arginine
Animals
Opossums
Nitric Oxide Synthase Type II
Baths

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research

Cite this

Cullen, Joseph J. ; Park, HyoJin ; Ephgrave, Kimberly S. ; Conklin, Jeffrey L. / Lipopolysaccharide temporarily impairs sphincter of Oddi motility. In: Nitric Oxide - Biology and Chemistry. 2001 ; Vol. 5, No. 6. pp. 547-554.
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Lipopolysaccharide temporarily impairs sphincter of Oddi motility. / Cullen, Joseph J.; Park, HyoJin; Ephgrave, Kimberly S.; Conklin, Jeffrey L.

In: Nitric Oxide - Biology and Chemistry, Vol. 5, No. 6, 01.01.2001, p. 547-554.

Research output: Contribution to journalArticle

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