Liprin-α is required for photoreceptor target selection in Drosophila

Kwang Min Choe, Saurabh Prakash, Ali Bright, Thomas R. Clandinin

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Classical cadherin-mediated interactions between axons and dendrites are critical to target selection and synapse assembly. However, the molecular mechanisms by which these interactions are controlled are incompletely understood. In the Drosophila visual system, N-cadherin is required in both photoreceptor (R cell) axons and their targets to mediate stabilizing interactions required for R cell target selection. Here we identify the scaffolding protein Liprin-α as a critical component in this process. We isolated mutations in Liprin-α in a genetic screen for mutations affecting the pattern of synaptic connections made by R1-R6 photoreceptors. Using eye-specific mosaics, we demonstrate a previously undescribed, axonal function for Liprin-α in target selection: Liprin-α is required to be cell-autonomous in all subtypes of R1-R6 cells for their axons to reach their targets. Because Liprin-α, the receptor tyrosine phosphatase LAR, and N-cadherin share qualitatively similar mutant phenotypes in R1-R6 cells and are coexpressed in R cells and their synaptic targets, we infer that these three genes act at the same step in the targeting process. However, unlike N-cadherin, neither Liprin-α nor LAR is required postsynaptically for R cells to project to their correct targets. Thus, these two proteins, unlike N-cadherin, are functionally asymmetric between axons and dendrites. We propose that the adhesive mechanisms that link pre- and postsynaptic cells before synapse formation may be differentially regulated in these two compartments.

Original languageEnglish
Pages (from-to)11601-11606
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number31
DOIs
Publication statusPublished - 2006 Aug 1

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Drosophila
Cadherins
Axons
Dendrites
Synapses
Class 2 Receptor-Like Protein Tyrosine Phosphatases
Photoreceptor Cells
Mutation
Adhesives
Proteins
Phenotype
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Liprin-α is required for photoreceptor target selection in Drosophila",
abstract = "Classical cadherin-mediated interactions between axons and dendrites are critical to target selection and synapse assembly. However, the molecular mechanisms by which these interactions are controlled are incompletely understood. In the Drosophila visual system, N-cadherin is required in both photoreceptor (R cell) axons and their targets to mediate stabilizing interactions required for R cell target selection. Here we identify the scaffolding protein Liprin-α as a critical component in this process. We isolated mutations in Liprin-α in a genetic screen for mutations affecting the pattern of synaptic connections made by R1-R6 photoreceptors. Using eye-specific mosaics, we demonstrate a previously undescribed, axonal function for Liprin-α in target selection: Liprin-α is required to be cell-autonomous in all subtypes of R1-R6 cells for their axons to reach their targets. Because Liprin-α, the receptor tyrosine phosphatase LAR, and N-cadherin share qualitatively similar mutant phenotypes in R1-R6 cells and are coexpressed in R cells and their synaptic targets, we infer that these three genes act at the same step in the targeting process. However, unlike N-cadherin, neither Liprin-α nor LAR is required postsynaptically for R cells to project to their correct targets. Thus, these two proteins, unlike N-cadherin, are functionally asymmetric between axons and dendrites. We propose that the adhesive mechanisms that link pre- and postsynaptic cells before synapse formation may be differentially regulated in these two compartments.",
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Liprin-α is required for photoreceptor target selection in Drosophila. / Choe, Kwang Min; Prakash, Saurabh; Bright, Ali; Clandinin, Thomas R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 31, 01.08.2006, p. 11601-11606.

Research output: Contribution to journalArticle

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