Live cell imaging based on surface plasmon-enhanced fluorescence microscopy using random nanostructures

Youngjin Oh, Wonju Lee, Taehwang Son, Sook Young Kim, Jeon Soo Shin, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Localized surface plasmon enhanced microscopy based on nanoislands of random spatial distribution was demonstrated for imaging live cells and molecular interactions. Nanoislands were produced without lithography by high temperature annealing under various processing conditions. The localization of near-field distribution that is associated with localized surface plasmon on metallic random nanoislands was analyzed theoretically and experimentally in comparison with periodic nanostructures. For experimental validation in live cell imaging, mouse macrophage-like cell line stained with Alexa Fluor 488 was prepared on nanoislands. The results suggest the possibility of attaining the imaging resolution on the order of 80 nm.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI
PublisherSPIE
ISBN (Print)9780819498670
DOIs
Publication statusPublished - 2014 Jan 1
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI - San Francisco, CA, United States
Duration: 2014 Feb 32014 Feb 6

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8954
ISSN (Print)1605-7422

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI
CountryUnited States
CitySan Francisco, CA
Period14/2/314/2/6

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Oh, Y., Lee, W., Son, T., Kim, S. Y., Shin, J. S., & Kim, D. (2014). Live cell imaging based on surface plasmon-enhanced fluorescence microscopy using random nanostructures. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI [89540E] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8954). SPIE. https://doi.org/10.1117/12.2038868