Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults: Isomers of C8 Health Project in China

Min Nian, Qing Qing Li, Michael Bloom, Zhengmin (Min) Qian, Kevin M. Syberg, Michael G. Vaughn, Si Quan Wang, Qi Wei, Mohammed Zeeshan, Namratha Gurram, Chu Chu, Jia Wang, Yan Peng Tian, Li Wen Hu, Kang Kang Liu, Bo Yi Yang, Ru Qing Liu, Dan Feng, Xiao Wen Zeng, Guang Hui Dong

Research output: Contribution to journalArticle

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Abstract

Exposure to chemicals may affect liver enzyme to increase the risk of liver diseases. Perfluoroalkyl acids (PFAAs) are one kind of persistent organic pollutants with hepatotoxic effect in organism. However, data is scarce to characterize the hepatotoxic effects of specific structural PFAA isomers in general population. To address this data gap, we evaluated the association between serum PFAAs concentration and liver function biomarkers in the Isomers of C8 Health Project in China. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to measure 18 serum PFAAs, except for linear and branched isomers of PFOA/PFOS, nine perfluorinated carboxylic acids (PFCAs) and two perfluorinated sulfonic acids (PFSAs) were also included, in 1605 adult residents of Shenyang, China. Values for nine serum liver function biomarkers were determined by full-automatic blood biochemical analyzer. Linear regression was used to evaluate associations between PFAAs and continuous liver function biomarkers and logistic regression to assess markers dichotomized per clinical reference intervals. Results indicated that serum PFAAs concentrations were associated with liver biomarker levels suggestive of hepatotoxicity, especially for liver cell injury. For example, a 1 ln-unit increase in total- perfluorooctanoic acid (PFOA) exposure was associated with a 7.4% [95% confidence interval (CI): 3.9%, 11.0%] higher alanine aminotransferase (ALT) level in serum. Interestingly, we observed association between branched PFAA isomers and liver biomarkers. For example, one ln-unit increase in branched perfluorooctane sulfonate (PFOS) isomers exposure was associated with a 4.3% increase in ALT level (95% CI: 1.2%, 7.4%) and a 33.0% increased odds of having abnormal ALT (95% CI: 5.0%, 67.0%). Also, we found that PFNA had positive association with ALT [(6.2%, 95% CI: 3.1%, 9.4%) and AST levels (2.5%, 95% CI: 0.5%, 4.5%)]. Logistic regression results showed that PFPeA, PFHxA, PFNA, PFDoDA, PFTrDA and PFTeDA had statistically association with abnormal prealbumin. Conclusively, our results support previous studies showing association between PFAAs exposure and liver function biomarkers. We found new evidence that branched PFAAs isomer exposure is associated with the risk of clinically relevant hepatocellular dysfunction.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalEnvironmental Research
Volume172
DOIs
Publication statusPublished - 2019 May

Fingerprint

Biomarkers
Isomers
Liver
biomarker
China
Health
Acids
acid
perfluorooctanoic acid
Alanine Transaminase
Association reactions
Confidence Intervals
confidence interval
serum
Serum
Logistics
sulfonate
Logistic Models
High Pressure Liquid Chromatography
health

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Environmental Science(all)

Cite this

Nian, Min ; Li, Qing Qing ; Bloom, Michael ; Qian, Zhengmin (Min) ; Syberg, Kevin M. ; Vaughn, Michael G. ; Wang, Si Quan ; Wei, Qi ; Zeeshan, Mohammed ; Gurram, Namratha ; Chu, Chu ; Wang, Jia ; Tian, Yan Peng ; Hu, Li Wen ; Liu, Kang Kang ; Yang, Bo Yi ; Liu, Ru Qing ; Feng, Dan ; Zeng, Xiao Wen ; Dong, Guang Hui. / Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults : Isomers of C8 Health Project in China. In: Environmental Research. 2019 ; Vol. 172. pp. 81-88.
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title = "Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults: Isomers of C8 Health Project in China",
abstract = "Exposure to chemicals may affect liver enzyme to increase the risk of liver diseases. Perfluoroalkyl acids (PFAAs) are one kind of persistent organic pollutants with hepatotoxic effect in organism. However, data is scarce to characterize the hepatotoxic effects of specific structural PFAA isomers in general population. To address this data gap, we evaluated the association between serum PFAAs concentration and liver function biomarkers in the Isomers of C8 Health Project in China. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to measure 18 serum PFAAs, except for linear and branched isomers of PFOA/PFOS, nine perfluorinated carboxylic acids (PFCAs) and two perfluorinated sulfonic acids (PFSAs) were also included, in 1605 adult residents of Shenyang, China. Values for nine serum liver function biomarkers were determined by full-automatic blood biochemical analyzer. Linear regression was used to evaluate associations between PFAAs and continuous liver function biomarkers and logistic regression to assess markers dichotomized per clinical reference intervals. Results indicated that serum PFAAs concentrations were associated with liver biomarker levels suggestive of hepatotoxicity, especially for liver cell injury. For example, a 1 ln-unit increase in total- perfluorooctanoic acid (PFOA) exposure was associated with a 7.4{\%} [95{\%} confidence interval (CI): 3.9{\%}, 11.0{\%}] higher alanine aminotransferase (ALT) level in serum. Interestingly, we observed association between branched PFAA isomers and liver biomarkers. For example, one ln-unit increase in branched perfluorooctane sulfonate (PFOS) isomers exposure was associated with a 4.3{\%} increase in ALT level (95{\%} CI: 1.2{\%}, 7.4{\%}) and a 33.0{\%} increased odds of having abnormal ALT (95{\%} CI: 5.0{\%}, 67.0{\%}). Also, we found that PFNA had positive association with ALT [(6.2{\%}, 95{\%} CI: 3.1{\%}, 9.4{\%}) and AST levels (2.5{\%}, 95{\%} CI: 0.5{\%}, 4.5{\%})]. Logistic regression results showed that PFPeA, PFHxA, PFNA, PFDoDA, PFTrDA and PFTeDA had statistically association with abnormal prealbumin. Conclusively, our results support previous studies showing association between PFAAs exposure and liver function biomarkers. We found new evidence that branched PFAAs isomer exposure is associated with the risk of clinically relevant hepatocellular dysfunction.",
author = "Min Nian and Li, {Qing Qing} and Michael Bloom and Qian, {Zhengmin (Min)} and Syberg, {Kevin M.} and Vaughn, {Michael G.} and Wang, {Si Quan} and Qi Wei and Mohammed Zeeshan and Namratha Gurram and Chu Chu and Jia Wang and Tian, {Yan Peng} and Hu, {Li Wen} and Liu, {Kang Kang} and Yang, {Bo Yi} and Liu, {Ru Qing} and Dan Feng and Zeng, {Xiao Wen} and Dong, {Guang Hui}",
year = "2019",
month = "5",
doi = "10.1016/j.envres.2019.02.013",
language = "English",
volume = "172",
pages = "81--88",
journal = "Environmental Research",
issn = "0013-9351",
publisher = "Academic Press Inc.",

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Nian, M, Li, QQ, Bloom, M, Qian, ZM, Syberg, KM, Vaughn, MG, Wang, SQ, Wei, Q, Zeeshan, M, Gurram, N, Chu, C, Wang, J, Tian, YP, Hu, LW, Liu, KK, Yang, BY, Liu, RQ, Feng, D, Zeng, XW & Dong, GH 2019, 'Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults: Isomers of C8 Health Project in China', Environmental Research, vol. 172, pp. 81-88. https://doi.org/10.1016/j.envres.2019.02.013

Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults : Isomers of C8 Health Project in China. / Nian, Min; Li, Qing Qing; Bloom, Michael; Qian, Zhengmin (Min); Syberg, Kevin M.; Vaughn, Michael G.; Wang, Si Quan; Wei, Qi; Zeeshan, Mohammed; Gurram, Namratha; Chu, Chu; Wang, Jia; Tian, Yan Peng; Hu, Li Wen; Liu, Kang Kang; Yang, Bo Yi; Liu, Ru Qing; Feng, Dan; Zeng, Xiao Wen; Dong, Guang Hui.

In: Environmental Research, Vol. 172, 05.2019, p. 81-88.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Liver function biomarkers disorder is associated with exposure to perfluoroalkyl acids in adults

T2 - Isomers of C8 Health Project in China

AU - Nian, Min

AU - Li, Qing Qing

AU - Bloom, Michael

AU - Qian, Zhengmin (Min)

AU - Syberg, Kevin M.

AU - Vaughn, Michael G.

AU - Wang, Si Quan

AU - Wei, Qi

AU - Zeeshan, Mohammed

AU - Gurram, Namratha

AU - Chu, Chu

AU - Wang, Jia

AU - Tian, Yan Peng

AU - Hu, Li Wen

AU - Liu, Kang Kang

AU - Yang, Bo Yi

AU - Liu, Ru Qing

AU - Feng, Dan

AU - Zeng, Xiao Wen

AU - Dong, Guang Hui

PY - 2019/5

Y1 - 2019/5

N2 - Exposure to chemicals may affect liver enzyme to increase the risk of liver diseases. Perfluoroalkyl acids (PFAAs) are one kind of persistent organic pollutants with hepatotoxic effect in organism. However, data is scarce to characterize the hepatotoxic effects of specific structural PFAA isomers in general population. To address this data gap, we evaluated the association between serum PFAAs concentration and liver function biomarkers in the Isomers of C8 Health Project in China. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to measure 18 serum PFAAs, except for linear and branched isomers of PFOA/PFOS, nine perfluorinated carboxylic acids (PFCAs) and two perfluorinated sulfonic acids (PFSAs) were also included, in 1605 adult residents of Shenyang, China. Values for nine serum liver function biomarkers were determined by full-automatic blood biochemical analyzer. Linear regression was used to evaluate associations between PFAAs and continuous liver function biomarkers and logistic regression to assess markers dichotomized per clinical reference intervals. Results indicated that serum PFAAs concentrations were associated with liver biomarker levels suggestive of hepatotoxicity, especially for liver cell injury. For example, a 1 ln-unit increase in total- perfluorooctanoic acid (PFOA) exposure was associated with a 7.4% [95% confidence interval (CI): 3.9%, 11.0%] higher alanine aminotransferase (ALT) level in serum. Interestingly, we observed association between branched PFAA isomers and liver biomarkers. For example, one ln-unit increase in branched perfluorooctane sulfonate (PFOS) isomers exposure was associated with a 4.3% increase in ALT level (95% CI: 1.2%, 7.4%) and a 33.0% increased odds of having abnormal ALT (95% CI: 5.0%, 67.0%). Also, we found that PFNA had positive association with ALT [(6.2%, 95% CI: 3.1%, 9.4%) and AST levels (2.5%, 95% CI: 0.5%, 4.5%)]. Logistic regression results showed that PFPeA, PFHxA, PFNA, PFDoDA, PFTrDA and PFTeDA had statistically association with abnormal prealbumin. Conclusively, our results support previous studies showing association between PFAAs exposure and liver function biomarkers. We found new evidence that branched PFAAs isomer exposure is associated with the risk of clinically relevant hepatocellular dysfunction.

AB - Exposure to chemicals may affect liver enzyme to increase the risk of liver diseases. Perfluoroalkyl acids (PFAAs) are one kind of persistent organic pollutants with hepatotoxic effect in organism. However, data is scarce to characterize the hepatotoxic effects of specific structural PFAA isomers in general population. To address this data gap, we evaluated the association between serum PFAAs concentration and liver function biomarkers in the Isomers of C8 Health Project in China. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to measure 18 serum PFAAs, except for linear and branched isomers of PFOA/PFOS, nine perfluorinated carboxylic acids (PFCAs) and two perfluorinated sulfonic acids (PFSAs) were also included, in 1605 adult residents of Shenyang, China. Values for nine serum liver function biomarkers were determined by full-automatic blood biochemical analyzer. Linear regression was used to evaluate associations between PFAAs and continuous liver function biomarkers and logistic regression to assess markers dichotomized per clinical reference intervals. Results indicated that serum PFAAs concentrations were associated with liver biomarker levels suggestive of hepatotoxicity, especially for liver cell injury. For example, a 1 ln-unit increase in total- perfluorooctanoic acid (PFOA) exposure was associated with a 7.4% [95% confidence interval (CI): 3.9%, 11.0%] higher alanine aminotransferase (ALT) level in serum. Interestingly, we observed association between branched PFAA isomers and liver biomarkers. For example, one ln-unit increase in branched perfluorooctane sulfonate (PFOS) isomers exposure was associated with a 4.3% increase in ALT level (95% CI: 1.2%, 7.4%) and a 33.0% increased odds of having abnormal ALT (95% CI: 5.0%, 67.0%). Also, we found that PFNA had positive association with ALT [(6.2%, 95% CI: 3.1%, 9.4%) and AST levels (2.5%, 95% CI: 0.5%, 4.5%)]. Logistic regression results showed that PFPeA, PFHxA, PFNA, PFDoDA, PFTrDA and PFTeDA had statistically association with abnormal prealbumin. Conclusively, our results support previous studies showing association between PFAAs exposure and liver function biomarkers. We found new evidence that branched PFAAs isomer exposure is associated with the risk of clinically relevant hepatocellular dysfunction.

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