Local injection of hyaluronic acid filler improves open gingival embrasure: Validation through a rat model

Soojin Pi, Yoon Jeong Choi, Soonshin Hwang, Dong Won Lee, Jong In Yook, Kyungho Kim, Chooryung Judi Chung

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: The open gingival embrasure, the so-called black triangle, is one of the unsolved dilemmas in adult dentistry. Although various techniques have been introduced to improve black triangles, the lack of reproducible experimental models has prevented development of successful protocols to regenerate or to compensate loss of the interdental papilla. Therefore, the objective of this study is first to develop a reliable animal model of open gingival embrasure and then to validate a minimally invasive injection technique using hyaluronic acid (HA) filler to augment the interdental papilla. Methods: To reproduce open gingival embrasure in rats, rapid space opening was induced between the mandibular incisors by attachment of a compression spring. Loss of interdental papilla height was morphologically evaluated and calculated using standardized serial photographs, microcomputed tomography, and histologic sections. Afterward, HA fillers or phosphate-buffered saline (PBS) was locally injected, and changes in the interdental papilla were evaluated. Results: After 7 days of space opening, the margin of the interdental papilla between the mandibular incisors gradually became irregular and flat, indicating a condition similar to the open gingival embrasure (P <0.05). Local injection of HA filler induced an augmentation effect of the interdental papilla compared with injection of PBS (P <0.05). Interdental papilla became convex, and inner granules containing HA were detected within the submucosal layer after its injection. Conclusions: Open gingival embrasure was reproduced reliably in vivo. Local injection of HA filler was validated as a meaningful minimally invasive procedure to improve open gingival embrasure.

Original languageEnglish
Pages (from-to)1221-1230
Number of pages10
JournalJournal of Periodontology
Volume88
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1

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Gingiva
Hyaluronic Acid
Injections
Incisor
Phosphates
X-Ray Microtomography
Dentistry
Theoretical Models
Animal Models

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Pi, Soojin ; Choi, Yoon Jeong ; Hwang, Soonshin ; Lee, Dong Won ; Yook, Jong In ; Kim, Kyungho ; Chung, Chooryung Judi. / Local injection of hyaluronic acid filler improves open gingival embrasure : Validation through a rat model. In: Journal of Periodontology. 2017 ; Vol. 88, No. 11. pp. 1221-1230.
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abstract = "Background: The open gingival embrasure, the so-called black triangle, is one of the unsolved dilemmas in adult dentistry. Although various techniques have been introduced to improve black triangles, the lack of reproducible experimental models has prevented development of successful protocols to regenerate or to compensate loss of the interdental papilla. Therefore, the objective of this study is first to develop a reliable animal model of open gingival embrasure and then to validate a minimally invasive injection technique using hyaluronic acid (HA) filler to augment the interdental papilla. Methods: To reproduce open gingival embrasure in rats, rapid space opening was induced between the mandibular incisors by attachment of a compression spring. Loss of interdental papilla height was morphologically evaluated and calculated using standardized serial photographs, microcomputed tomography, and histologic sections. Afterward, HA fillers or phosphate-buffered saline (PBS) was locally injected, and changes in the interdental papilla were evaluated. Results: After 7 days of space opening, the margin of the interdental papilla between the mandibular incisors gradually became irregular and flat, indicating a condition similar to the open gingival embrasure (P <0.05). Local injection of HA filler induced an augmentation effect of the interdental papilla compared with injection of PBS (P <0.05). Interdental papilla became convex, and inner granules containing HA were detected within the submucosal layer after its injection. Conclusions: Open gingival embrasure was reproduced reliably in vivo. Local injection of HA filler was validated as a meaningful minimally invasive procedure to improve open gingival embrasure.",
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Local injection of hyaluronic acid filler improves open gingival embrasure : Validation through a rat model. / Pi, Soojin; Choi, Yoon Jeong; Hwang, Soonshin; Lee, Dong Won; Yook, Jong In; Kim, Kyungho; Chung, Chooryung Judi.

In: Journal of Periodontology, Vol. 88, No. 11, 01.11.2017, p. 1221-1230.

Research output: Contribution to journalArticle

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AU - Pi, Soojin

AU - Choi, Yoon Jeong

AU - Hwang, Soonshin

AU - Lee, Dong Won

AU - Yook, Jong In

AU - Kim, Kyungho

AU - Chung, Chooryung Judi

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N2 - Background: The open gingival embrasure, the so-called black triangle, is one of the unsolved dilemmas in adult dentistry. Although various techniques have been introduced to improve black triangles, the lack of reproducible experimental models has prevented development of successful protocols to regenerate or to compensate loss of the interdental papilla. Therefore, the objective of this study is first to develop a reliable animal model of open gingival embrasure and then to validate a minimally invasive injection technique using hyaluronic acid (HA) filler to augment the interdental papilla. Methods: To reproduce open gingival embrasure in rats, rapid space opening was induced between the mandibular incisors by attachment of a compression spring. Loss of interdental papilla height was morphologically evaluated and calculated using standardized serial photographs, microcomputed tomography, and histologic sections. Afterward, HA fillers or phosphate-buffered saline (PBS) was locally injected, and changes in the interdental papilla were evaluated. Results: After 7 days of space opening, the margin of the interdental papilla between the mandibular incisors gradually became irregular and flat, indicating a condition similar to the open gingival embrasure (P <0.05). Local injection of HA filler induced an augmentation effect of the interdental papilla compared with injection of PBS (P <0.05). Interdental papilla became convex, and inner granules containing HA were detected within the submucosal layer after its injection. Conclusions: Open gingival embrasure was reproduced reliably in vivo. Local injection of HA filler was validated as a meaningful minimally invasive procedure to improve open gingival embrasure.

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