Local injection of RANKL facilitates tooth movement and alveolar bone remodelling

Research output: Contribution to journalArticle

Abstract

Objectives: To investigate the effect of local injection of receptor activator of nuclear factor kappa B ligand (RANKL) on experimental tooth movement and subsequent alveolar bone remodelling in mice. Materials and Methods: Sixty mice were randomised to receive daily local RANKL or phosphate-buffered saline injections in the buccal premaxillary bone for 14 of 21 days of incisor movement, followed by a 21-day retention period. Five mice from each group were euthanised on days 0, 3, 7, 14, 21 and 42, and specimens were prepared for haematoxylin and eosin, tartrate-resistant acid phosphatase and immunohistochemical staining. Five mice from each group were subjected to serial microcomputed tomography until day 42 for tooth movement and bone volume quantification. Results: The experimental group showed significantly greater tooth movement and bone volume reduction on days 14 and 21; an increased osteoclast number on days 3, 7, 14 and 21; and no difference on day 42. Higher RANKL expression was observed on days 7 and 14, with remarkable alkaline phosphatase activity. No significant systemic changes were observed. Conclusion: Local RANKL injection leads to increased osteoclastic activity and facilitates tooth movement, followed by subsequent alveolar bone formation; this implies a reversible transitional acceleration of bone resorption.

Original languageEnglish
Pages (from-to)550-560
Number of pages11
JournalOral Diseases
Volume25
Issue number2
DOIs
Publication statusPublished - 2019 Mar

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RANK Ligand
Tooth Movement Techniques
Bone Remodeling
Injections
Zygoma
Bone and Bones
X-Ray Microtomography
Osteoclasts
Incisor
Hematoxylin
Eosine Yellowish-(YS)
Bone Resorption
Osteogenesis
Alkaline Phosphatase
Phosphates
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

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title = "Local injection of RANKL facilitates tooth movement and alveolar bone remodelling",
abstract = "Objectives: To investigate the effect of local injection of receptor activator of nuclear factor kappa B ligand (RANKL) on experimental tooth movement and subsequent alveolar bone remodelling in mice. Materials and Methods: Sixty mice were randomised to receive daily local RANKL or phosphate-buffered saline injections in the buccal premaxillary bone for 14 of 21 days of incisor movement, followed by a 21-day retention period. Five mice from each group were euthanised on days 0, 3, 7, 14, 21 and 42, and specimens were prepared for haematoxylin and eosin, tartrate-resistant acid phosphatase and immunohistochemical staining. Five mice from each group were subjected to serial microcomputed tomography until day 42 for tooth movement and bone volume quantification. Results: The experimental group showed significantly greater tooth movement and bone volume reduction on days 14 and 21; an increased osteoclast number on days 3, 7, 14 and 21; and no difference on day 42. Higher RANKL expression was observed on days 7 and 14, with remarkable alkaline phosphatase activity. No significant systemic changes were observed. Conclusion: Local RANKL injection leads to increased osteoclastic activity and facilitates tooth movement, followed by subsequent alveolar bone formation; this implies a reversible transitional acceleration of bone resorption.",
author = "Chengri Li and Chung, {Chooryung J.} and Hwang, {Chung Ju} and Lee, {Kee Joon}",
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Local injection of RANKL facilitates tooth movement and alveolar bone remodelling. / Li, Chengri; Chung, Chooryung J.; Hwang, Chung Ju; Lee, Kee Joon.

In: Oral Diseases, Vol. 25, No. 2, 03.2019, p. 550-560.

Research output: Contribution to journalArticle

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