Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo

J. E. Jung, M. J. Song, S. Shin, Y. J. Choi, Kyungho Kim, Chooryung Judi Chung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2′-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. Setting and Sample Population: Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). Material and Methods: Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice. Results: Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles. Conclusion: The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalOrthodontics and Craniofacial Research
Volume20
Issue number1
DOIs
Publication statusPublished - 2017 Feb 1

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Regeneration
Muscles
Injections
Bicuspid
Masseter Muscle
decitabine
Epigenomics
Cardiotoxins
Myogenin
Desmin
Skeletal Muscle Fibers
Orthodontics
Green Fluorescent Proteins
Skeletal Muscle
Collagen
Gels
DNA
Wounds and Injuries
Therapeutics
Population

All Science Journal Classification (ASJC) codes

  • Orthodontics
  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

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title = "Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo",
abstract = "Objectives: To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2′-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. Setting and Sample Population: Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). Material and Methods: Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice. Results: Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles. Conclusion: The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo.",
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Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo. / Jung, J. E.; Song, M. J.; Shin, S.; Choi, Y. J.; Kim, Kyungho; Chung, Chooryung Judi.

In: Orthodontics and Craniofacial Research, Vol. 20, No. 1, 01.02.2017, p. 35-43.

Research output: Contribution to journalArticle

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AU - Song, M. J.

AU - Shin, S.

AU - Choi, Y. J.

AU - Kim, Kyungho

AU - Chung, Chooryung Judi

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