Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae

K. Y. Jeong, I. Y. Lee, H. I. Ree, C. S. Hong, T. S. Yong

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: House dust mite derived materials are known to be the most potent agent inducing allergic diseases. Localization of Der f 2 was attempted to specify the sites and concentrations of Der f 2 within the mite, which may indicate the importance of secreted materials and nonexcreted body components as allergen sources. Methods: Serial cryostat sections of embedded live mites and the fecal pellets, collected by brush, were immunoprobed using monoclonal antibody (mAb) 2F38 raised against recombinant (r) Der f 2. Results: Highest concentrations were found in the anterior midgut, implying that this is the site of Der f 2 synthesis and secretion. Digestive material and defecated fecal pellets were also labeled with mAb. Conclusions: Our results show that the major allergen, Der f 2, found in the house dust mite D. farinae is derived from the digestive tract, and is concentrated in the feces.

Original languageEnglish
Pages (from-to)729-731
Number of pages3
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume57
Issue number8
DOIs
Publication statusPublished - 2002 Jan 1

Fingerprint

Dermatophagoides farinae
Pyroglyphidae
Mites
Monoclonal Antibodies
Feces
Allergens
Gastrointestinal Tract
Dermatophagoides farinae antigen f 2

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

@article{05715992f0c7453896f90c976973ceca,
title = "Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae",
abstract = "Background: House dust mite derived materials are known to be the most potent agent inducing allergic diseases. Localization of Der f 2 was attempted to specify the sites and concentrations of Der f 2 within the mite, which may indicate the importance of secreted materials and nonexcreted body components as allergen sources. Methods: Serial cryostat sections of embedded live mites and the fecal pellets, collected by brush, were immunoprobed using monoclonal antibody (mAb) 2F38 raised against recombinant (r) Der f 2. Results: Highest concentrations were found in the anterior midgut, implying that this is the site of Der f 2 synthesis and secretion. Digestive material and defecated fecal pellets were also labeled with mAb. Conclusions: Our results show that the major allergen, Der f 2, found in the house dust mite D. farinae is derived from the digestive tract, and is concentrated in the feces.",
author = "Jeong, {K. Y.} and Lee, {I. Y.} and Ree, {H. I.} and Hong, {C. S.} and Yong, {T. S.}",
year = "2002",
month = "1",
day = "1",
doi = "10.1034/j.1398-9995.2002.23623.x",
language = "English",
volume = "57",
pages = "729--731",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley-Blackwell",
number = "8",

}

Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae. / Jeong, K. Y.; Lee, I. Y.; Ree, H. I.; Hong, C. S.; Yong, T. S.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 57, No. 8, 01.01.2002, p. 729-731.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae

AU - Jeong, K. Y.

AU - Lee, I. Y.

AU - Ree, H. I.

AU - Hong, C. S.

AU - Yong, T. S.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Background: House dust mite derived materials are known to be the most potent agent inducing allergic diseases. Localization of Der f 2 was attempted to specify the sites and concentrations of Der f 2 within the mite, which may indicate the importance of secreted materials and nonexcreted body components as allergen sources. Methods: Serial cryostat sections of embedded live mites and the fecal pellets, collected by brush, were immunoprobed using monoclonal antibody (mAb) 2F38 raised against recombinant (r) Der f 2. Results: Highest concentrations were found in the anterior midgut, implying that this is the site of Der f 2 synthesis and secretion. Digestive material and defecated fecal pellets were also labeled with mAb. Conclusions: Our results show that the major allergen, Der f 2, found in the house dust mite D. farinae is derived from the digestive tract, and is concentrated in the feces.

AB - Background: House dust mite derived materials are known to be the most potent agent inducing allergic diseases. Localization of Der f 2 was attempted to specify the sites and concentrations of Der f 2 within the mite, which may indicate the importance of secreted materials and nonexcreted body components as allergen sources. Methods: Serial cryostat sections of embedded live mites and the fecal pellets, collected by brush, were immunoprobed using monoclonal antibody (mAb) 2F38 raised against recombinant (r) Der f 2. Results: Highest concentrations were found in the anterior midgut, implying that this is the site of Der f 2 synthesis and secretion. Digestive material and defecated fecal pellets were also labeled with mAb. Conclusions: Our results show that the major allergen, Der f 2, found in the house dust mite D. farinae is derived from the digestive tract, and is concentrated in the feces.

UR - http://www.scopus.com/inward/record.url?scp=0036338572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036338572&partnerID=8YFLogxK

U2 - 10.1034/j.1398-9995.2002.23623.x

DO - 10.1034/j.1398-9995.2002.23623.x

M3 - Article

C2 - 12121193

AN - SCOPUS:0036338572

VL - 57

SP - 729

EP - 731

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 8

ER -