Abstract
Background/Aim: To explore the molecular mechanism and clinical significance of a newly identified lncRNA LOC285194 in epithelial ovarian cancer (EOC). Materials and Methods: LOC285194 transcript levels were analyzed in EOC cells compared to normal cells. Small interfering RNAs were used to suppress LOC285194 expression. Levels of apoptosis-related proteins were determined by western blot. LOC285194 expression in ovarian cancer and non-tumor tissues were compared with clinicopathologic and survival data. Results: Knockdown of LOC285194 decreased cell migration and proliferation, enhanced reactive oxygen species production and resulted in increased levels of proteins of the extrinsic apoptotic signaling pathway. LOC285194 expression level was higher in ovarian cancer tissues compared to control. Overall survival was significantly shorter in patients with high LOC285194 expression. Lymph node metastasis and high LOC285194 expression were significant prognostic factors of mortality (HR=4.614 and 5.880; p=0.026 and p=0.002, respectively). Conclusion: LOC285194 can promote the progression of EOC via an anti-apoptotic mechanism. It may serve as a novel biomarker for predicting prognosis of EOC.
| Original language | English |
|---|---|
| Pages (from-to) | 121-131 |
| Number of pages | 11 |
| Journal | In Vivo |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2022 Feb |
Bibliographical note
Funding Information:Cell lines and cell culture. Human epithelial ovarian cancer (EOC) cell lines A2780 was purchased from the European Collection of Cell Cultures (ECACC, Sigma-Aldrich, St. Louis, MO, USA) and cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (pen/strep, Gibco). SKOV3 cell line was obtained from the Korean Cell Line Bank (KCLB, Seoul, Republic of Korea). OVCA433, OVCA429, and TOV112D cell lines were provided by the Korea Gynecologic Cancer Bank through the Bio & Medical Technology Development Program of the Minister of Science, Information and Communication Technology and Future Planning (MSIP), Korea and cultured in Dulbecco’s modified Eagle’s medium. These cell lines were maintained at 37˚C with 5% CO2.The culture medium was replaced with fresh medium every 2-3 days. The passage number of cells was <20 in all experiments.
Funding Information:
This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (grant number NRF-2018R1D1A1B07049578).
Publisher Copyright:
© 2022 International Institute of Anticancer Research. All rights reserved.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology
- Cancer Research
