Long-term antibacterial performance and bioactivity of plasma-engineered Ag-NPs/TiO2 nanotubes for bio-implants

Soo Hyuk Uhm, Jae Sung Kwon, Doo Hoon Song, Eun Jung Lee, Won Seok Jeong, Seunghan Oh, Kyoung Nam Kim, Eun Ha Choi, Kwang Mahn Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We prepared TiO2 nanotubes (NT) on commercially pure titanium (cp-Ti) substrate by plasma electrolyte oxidation and adapted magnetron sputtering for incorporation of Ag-nanoparticles (Ag-NPs) onto the nanotubes (Ag-NPs/TiO2 nanotube). Power input to the Ag target per unit time was varied (5, 10, 15 W/cm2) to fabricate different shapes of Agnanoparticles onto the nanotubes while net energy input was fixed by maintaining a constant total sputter time (30, 15, 10 s, respectively). For investigation of experimental samples' characteristics, FE-SEM, TEM, EDS, XRD, XPS, SPM analysis and contact angles measurement was carried out. Through these characterization, plasma engineered Ag-NPs was successfully formed on/in the entire nanotube structure. In terms of antibacterial ability, plasma engineered Ag-NPs/TiO2 nanotubes samples significantly reduced S. aureus colony numbers compared with control. Also, simulated body fluid immersion tests with hydroxyapatite showed ion precipitation onto the surface of all experimental groups, confirmed by XRD and EDS analysis. However, plasma engineered Ag-NPs/TiO2 nanotubes groups were not cytotoxic. Furthermore, MC3T3-E1 cells were cultured on Ag-NPs/TiO2 nanotubes groups to evaluate the effect of nanostructured surface on cell functionality such as a cell proliferation and ALP activity. Ag-NPs/TiO2 nanotubes have both biocompatible and antibacterial characteristics.

Original languageEnglish
Pages (from-to)1890-1906
Number of pages17
JournalJournal of Biomedical Nanotechnology
Volume12
Issue number10
DOIs
Publication statusPublished - 2016 Oct

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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