Long-term viability of transplanted hybrid cellular spheroids within chondrocyte sheets

J. I. Lee, J. Y. Kim, H. W. Kim, S. J. Bae, D. J. Joo, K. H. Huh, Y. H. Fang, J. H. Jeong, M. S. Kim, Y. S. Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Encapsulation of transplanted cells within an immunoisolating membrane may provide a new strategy for protecting these cells from recipient immune responses without the use of immunosuppressive drugs. We have previously reported a novel concept of immunoisolation and immunodelusion using recipient cells instead of traditional artificial materials. We developed a chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas (CSI-BAP) that would enable transplantation of cells across allogeneic and xenogeneic barriers without the cells being recognized as donor cells and without the need for immunosuppression. Recently, we have constructed hybrid cellular spheroids (HCSs) containing cells from two different cell lines (RIN-5F, an insulin-secreting cell line, and Hep-G2, a hepatocellular carcinoma cell line) to enhance the function and biocompatibility of the HCSs. These HCSs were then encapsulated with multiple layers of chondrocyte sheets obtained from the auricular cartilage of Sprague-Dawley (SD) rats. The in vitro ability of the CSI-BAP to secrete insulin was tested before transplantation. Histological evaluation of CSI-BAP chondrocyte microencapsulated immunoisolated islet morphology and viability of allogeneic or xenogeneic cell lines was performed 100 days after the CSI-BAP was transplanted into SD rats. Morphological evaluations revealed good viability of the islets and progression of islet encapsulation. In vitro insulin secretion from the CSI-BAP was well maintained. Additionally, insulin and albumin secretion from the CSI-BAP was confirmed by in vivo immunohistochemical examination. Moreover, the cell lines transplanted into the subcutaneous space in the form of HCSs within the chondrocyte sheets showed good viability of more than 100 days and sustained insulin and albumin secreting ability.

Original languageEnglish
Pages (from-to)1162-1165
Number of pages4
JournalTransplantation Proceedings
Issue number4
Publication statusPublished - 2012 May

Bibliographical note

Funding Information:
Supported by the faculty research fund of Konkuk University in 2010.

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation


Dive into the research topics of 'Long-term viability of transplanted hybrid cellular spheroids within chondrocyte sheets'. Together they form a unique fingerprint.

Cite this