Low binding affinity and reduced complement-dependent cell death efficacy of ofatumumab produced using a plant system (Nicotiana benthamiana L.)

Narae Jin, Jin Won Lee, Woon Heo, Moon Young Ryu, Min Kyung So, Byoung Joon Ko, Hye Yeon Kim, Sei Mee Yoon, Jinu Lee, Joo Young Kim, Woo Taek Kim

Research output: Contribution to journalArticle

Abstract

The plant protein production system is a platform that can not only reduce production costs but also produce monoclonal antibodies that do not have the risk of residual proteins from the host. However, due to the difference between post-translational processes in plants and animals, there may be a modification in the Fab region of the monoclonal antibody produced in the plant; thus, it is necessary to compare the antigen affinity of this antibody with that of the prototype. In this study, ofatumumab, a fully human anti-CD20 IgG1κ monoclonal antibody used for its non-cross resistance to rituximab, was expressed in Nicotiana benthamiana, and its affinities and efficacies were compared with those of native ofatumumab produced from CHO cells. Two forms of plant ofatumumab (with or without HDEL-tag) were generated and their production yields were compared. The HDEL-tagged ofatumumab was more expressed in plants than the form without HDEL-tag. The specificity of the target recognition of plant-derived ofatumumab was confirmed by mCherry-CD20-expressing HEK cells via immuno-staining, and the capping of CD20 after ofatumumab binding was also confirmed using Ramos B cells. In the functional equivalence tests, the binding affinities and complement-dependent cell cytotoxicity efficacy of plant-ofatumumab-HDEL and plant-ofatumumab without HDEL were significantly reduced compared to those of CHO-derived ofatumumab. Therefore, we suggest that although ofatumumab is not a good candidate as a template for plant-derived monoclonal antibodies because of its decreased affinity when produced in plants, it is an interesting target to study the differences between post-translational modifications in mammals and plants.

Original languageEnglish
Pages (from-to)34-41
Number of pages8
JournalProtein Expression and Purification
Volume159
DOIs
Publication statusPublished - 2019 Jul 1

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Tobacco
Cell Death
Monoclonal Antibodies
Plantibodies
ofatumumab
Plant Proteins
Antibody Affinity
CHO Cells
Post Translational Protein Processing
Mammals
B-Lymphocytes
Immunoglobulin G
Staining and Labeling
Antigens
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Jin, Narae ; Lee, Jin Won ; Heo, Woon ; Ryu, Moon Young ; So, Min Kyung ; Ko, Byoung Joon ; Kim, Hye Yeon ; Yoon, Sei Mee ; Lee, Jinu ; Kim, Joo Young ; Kim, Woo Taek. / Low binding affinity and reduced complement-dependent cell death efficacy of ofatumumab produced using a plant system (Nicotiana benthamiana L.). In: Protein Expression and Purification. 2019 ; Vol. 159. pp. 34-41.
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Low binding affinity and reduced complement-dependent cell death efficacy of ofatumumab produced using a plant system (Nicotiana benthamiana L.). / Jin, Narae; Lee, Jin Won; Heo, Woon; Ryu, Moon Young; So, Min Kyung; Ko, Byoung Joon; Kim, Hye Yeon; Yoon, Sei Mee; Lee, Jinu; Kim, Joo Young; Kim, Woo Taek.

In: Protein Expression and Purification, Vol. 159, 01.07.2019, p. 34-41.

Research output: Contribution to journalArticle

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AU - Kim, Woo Taek

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