Lps-induced vascular endothelial growth factor expression in rat lung pericytes

Chang Oh Kim, Ae Jung Huh, Myung Soo Kim, Bum Sik Chin, Sang Hoon Han, Suk Hoon Choi, Su Jin Jeong, Hee Kyung Choi, JunYong Choi, Young Goo Song, June Myung Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) is a potent angiogenic and vascular permeability factor. Recent studies have shown that the VEGF levels increase in several cell types, for example, macrophages and smooth muscle cells after LPS stimulation, suggesting that it is important in the initiation and development of sepsis. In particular, LPS-regulated contractility in lung pericytes may play an important role in mediating pulmonary microvascular fluid hemodynamics during sepsis. This study investigated the production of VEGF by rat lung pericytes in response to LPS. LPS was found to enhance VEGF mRNA expression in a concentration-dependent manner peaking 2 h after stimulation in pericytes. Vascular endothelial growth factor protein levels in conditioned medium and in cell lysate also increased on increasing LPS and peaked after 24 to 48 h. LPS also significantly augmented iNOS expression in lung pericytes within 6 h. However, iNOS mRNA induction occurred later than LPS-induced VEGF mRNA increases. Interestingly, attempted inhibition with nuclear factor-κB or tyrosine kinase did not suppress LPS-induced augmented VEGF mRNA expression in lung pericytes, although both inhibitors markedly inhibited LPS-induced iNOS mRNA expression. SB203580, a p38 MAP kinase inhibitor, repressed LPS-induced VEGF mRNA expression. Furthermore, LPS stimulated a rapid and sustained phosphorylation of p38 MAP kinase. These results show that pericytes produce VEGF in response to LPS stimulation, and that this may be partly mediated by the p38 MAP kinase pathway. More research should be done to establish the regulation of capillary hemodynamics and identify mechanisms of their regulation.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalShock
Volume30
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1

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Pericytes
Vascular Endothelial Growth Factor A
Lung
Messenger RNA
p38 Mitogen-Activated Protein Kinases
Sepsis
Hemodynamics
MAP Kinase Signaling System
Conditioned Culture Medium
Protein-Tyrosine Kinases
Smooth Muscle Myocytes
Macrophages
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Kim, C. O., Huh, A. J., Kim, M. S., Chin, B. S., Han, S. H., Choi, S. H., ... Kim, J. M. (2008). Lps-induced vascular endothelial growth factor expression in rat lung pericytes. Shock, 30(1), 92-97. https://doi.org/10.1097/SHK.0b013e31815d19ad
Kim, Chang Oh ; Huh, Ae Jung ; Kim, Myung Soo ; Chin, Bum Sik ; Han, Sang Hoon ; Choi, Suk Hoon ; Jeong, Su Jin ; Choi, Hee Kyung ; Choi, JunYong ; Song, Young Goo ; Kim, June Myung. / Lps-induced vascular endothelial growth factor expression in rat lung pericytes. In: Shock. 2008 ; Vol. 30, No. 1. pp. 92-97.
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Kim, CO, Huh, AJ, Kim, MS, Chin, BS, Han, SH, Choi, SH, Jeong, SJ, Choi, HK, Choi, J, Song, YG & Kim, JM 2008, 'Lps-induced vascular endothelial growth factor expression in rat lung pericytes', Shock, vol. 30, no. 1, pp. 92-97. https://doi.org/10.1097/SHK.0b013e31815d19ad

Lps-induced vascular endothelial growth factor expression in rat lung pericytes. / Kim, Chang Oh; Huh, Ae Jung; Kim, Myung Soo; Chin, Bum Sik; Han, Sang Hoon; Choi, Suk Hoon; Jeong, Su Jin; Choi, Hee Kyung; Choi, JunYong; Song, Young Goo; Kim, June Myung.

In: Shock, Vol. 30, No. 1, 01.07.2008, p. 92-97.

Research output: Contribution to journalArticle

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AU - Kim, Chang Oh

AU - Huh, Ae Jung

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AU - Choi, Suk Hoon

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AU - Song, Young Goo

AU - Kim, June Myung

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